Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2289914..2290568 Replicon chromosome
Accession NZ_CP020339
Organism Shigella flexneri 4c strain 0702

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag BS653_RS12630 Protein ID WP_000244767.1
Coordinates 2289914..2290321 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag BS653_RS12635 Protein ID WP_000354052.1
Coordinates 2290302..2290568 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS653_RS12610 (2285871) 2285871..2287604 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
BS653_RS12615 (2287610) 2287610..2288320 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BS653_RS12620 (2288345) 2288345..2289241 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
BS653_RS12625 (2289353) 2289353..2289874 + 522 WP_001055867.1 flavodoxin FldB -
BS653_RS12630 (2289914) 2289914..2290321 - 408 WP_000244767.1 protein YgfX Toxin
BS653_RS12635 (2290302) 2290302..2290568 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
BS653_RS12640 (2290811) 2290811..2291791 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BS653_RS12645 (2291987) 2291987..2292646 - 660 WP_000250274.1 hemolysin III family protein -
BS653_RS12650 (2292810) 2292810..2293120 - 311 Protein_2306 N(4)-acetylcytidine aminohydrolase -
BS653_RS12655 (2293165) 2293165..2294598 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
BS653_RS12660 (2294655) 2294655..2295398 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T74300 WP_000244767.1 NZ_CP020339:c2290321-2289914 [Shigella flexneri 4c]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T74300 NZ_CP020339:c2290321-2289914 [Shigella flexneri 4c]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT74300 WP_000354052.1 NZ_CP020339:c2290568-2290302 [Shigella flexneri 4c]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74300 NZ_CP020339:c2290568-2290302 [Shigella flexneri 4c]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References