Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 402785..403580 | Replicon | chromosome |
| Accession | NZ_CP020339 | ||
| Organism | Shigella flexneri 4c strain 0702 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | Q93EZ7 |
| Locus tag | BS653_RS02215 | Protein ID | WP_000854917.1 |
| Coordinates | 403206..403580 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D2ACA1 |
| Locus tag | BS653_RS02210 | Protein ID | WP_001280958.1 |
| Coordinates | 402785..403159 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BS653_RS02165 (398122) | 398122..398244 | + | 123 | Protein_393 | transcriptional regulator | - |
| BS653_RS02170 (398222) | 398222..398634 | - | 413 | Protein_394 | tetracycline resistance transcriptional repressor TetR(B) | - |
| BS653_RS02175 (398674) | 398674..399371 | + | 698 | WP_095033700.1 | IS1-like element IS1B family transposase | - |
| BS653_RS27325 (399554) | 399554..399817 | + | 264 | WP_000466628.1 | DNA polymerase III subunit gamma/tau | - |
| BS653_RS26080 (399937) | 399937..400110 | + | 174 | WP_000088744.1 | DUF905 family protein | - |
| BS653_RS02185 (400210) | 400210..401031 | + | 822 | WP_001234584.1 | DUF932 domain-containing protein | - |
| BS653_RS26090 (401373) | 401373..401846 | + | 474 | WP_005011597.1 | antirestriction protein | - |
| BS653_RS02195 (401862) | 401862..402338 | + | 477 | WP_005011624.1 | RadC family protein | - |
| BS653_RS02200 (402401) | 402401..402622 | + | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| BS653_RS02210 (402785) | 402785..403159 | + | 375 | WP_001280958.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| BS653_RS02215 (403206) | 403206..403580 | + | 375 | WP_000854917.1 | TA system toxin CbtA family protein | Toxin |
| BS653_RS02220 (403577) | 403577..404068 | + | 492 | WP_000976828.1 | DUF5983 family protein | - |
| BS653_RS02225 (404080) | 404080..404277 | + | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| BS653_RS02230 (404362) | 404362..404922 | + | 561 | Protein_406 | DUF4942 domain-containing protein | - |
| BS653_RS02235 (404994) | 404994..405998 | + | 1005 | WP_001179707.1 | IS110-like element ISSfl8 family transposase | - |
| BS653_RS02240 (406280) | 406280..406561 | + | 282 | Protein_408 | DUF4942 domain-containing protein | - |
| BS653_RS02250 (407033) | 407033..407971 | + | 939 | Protein_409 | glyoxylate/hydroxypyruvate reductase GhrA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | tet(B) / ant(3'')-Ia / blaOXA-1 / catA1 | csgG / csgF / csgE / csgD / csgB | 362025..413494 | 51469 | |
| - | flank | IS/Tn | - | - | 404994..405998 | 1004 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14145.22 Da Isoelectric Point: 7.7761
>T74286 WP_000854917.1 NZ_CP020339:403206-403580 [Shigella flexneri 4c]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T74286 NZ_CP020339:403206-403580 [Shigella flexneri 4c]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13793.62 Da Isoelectric Point: 6.4651
>AT74286 WP_001280958.1 NZ_CP020339:402785-403159 [Shigella flexneri 4c]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT74286 NZ_CP020339:402785-403159 [Shigella flexneri 4c]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q93EZ7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0K4PAV2 |