Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3120863..3121517 Replicon chromosome
Accession NZ_CP020336
Organism Shigella flexneri 4c strain 1602

Toxin (Protein)


Gene name cptA Uniprot ID Q0T101
Locus tag BS654_RS17140 Protein ID WP_000244767.1
Coordinates 3120863..3121270 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID D2A730
Locus tag BS654_RS17145 Protein ID WP_000354052.1
Coordinates 3121251..3121517 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS654_RS17120 (3116820) 3116820..3118553 - 1734 WP_000813187.1 single-stranded-DNA-specific exonuclease RecJ -
BS654_RS17125 (3118559) 3118559..3119269 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BS654_RS17130 (3119294) 3119294..3120190 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
BS654_RS17135 (3120302) 3120302..3120823 + 522 WP_001055867.1 flavodoxin FldB -
BS654_RS17140 (3120863) 3120863..3121270 - 408 WP_000244767.1 protein YgfX Toxin
BS654_RS17145 (3121251) 3121251..3121517 - 267 WP_000354052.1 FAD assembly factor SdhE Antitoxin
BS654_RS17150 (3121760) 3121760..3122740 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BS654_RS17155 (3122936) 3122936..3123595 - 660 WP_000250274.1 hemolysin III family protein -
BS654_RS17160 (3123759) 3123759..3124069 - 311 Protein_3130 N(4)-acetylcytidine aminohydrolase -
BS654_RS17165 (3124114) 3124114..3125547 + 1434 WP_005051722.1 6-phospho-beta-glucosidase BglA -
BS654_RS17170 (3125604) 3125604..3126347 - 744 WP_000951957.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15978.91 Da        Isoelectric Point: 10.9373

>T74277 WP_000244767.1 NZ_CP020336:c3121270-3120863 [Shigella flexneri 4c]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T74277 NZ_CP020336:c3121270-3120863 [Shigella flexneri 4c]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGTGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10519.05 Da        Isoelectric Point: 5.1599

>AT74277 WP_000354052.1 NZ_CP020336:c3121517-3121251 [Shigella flexneri 4c]
MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74277 NZ_CP020336:c3121517-3121251 [Shigella flexneri 4c]
ATGGACATTAACAACAAAGCCCGAATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGTCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATAATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TIU0

Antitoxin

Source ID Structure
AlphaFold DB D2A730

References