Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 1225634..1226429 | Replicon | chromosome |
| Accession | NZ_CP020336 | ||
| Organism | Shigella flexneri 4c strain 1602 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | Q93EZ7 |
| Locus tag | BS654_RS06655 | Protein ID | WP_000854917.1 |
| Coordinates | 1226055..1226429 (+) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | D2ACA1 |
| Locus tag | BS654_RS06650 | Protein ID | WP_001280958.1 |
| Coordinates | 1225634..1226008 (+) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BS654_RS06600 (1220971) | 1220971..1221093 | + | 123 | Protein_1209 | transcriptional regulator | - |
| BS654_RS06605 (1221071) | 1221071..1221483 | - | 413 | Protein_1210 | tetracycline resistance transcriptional repressor TetR(B) | - |
| BS654_RS06615 (1221523) | 1221523..1222220 | + | 698 | WP_095033700.1 | IS1-like element IS1B family transposase | - |
| BS654_RS28855 (1222403) | 1222403..1222666 | + | 264 | WP_000466628.1 | DNA polymerase III subunit gamma/tau | - |
| BS654_RS27535 (1222786) | 1222786..1222959 | + | 174 | WP_000088744.1 | DUF905 family protein | - |
| BS654_RS06625 (1223059) | 1223059..1223880 | + | 822 | WP_001234584.1 | DUF932 domain-containing protein | - |
| BS654_RS27545 (1224222) | 1224222..1224695 | + | 474 | WP_005011597.1 | antirestriction protein | - |
| BS654_RS06635 (1224711) | 1224711..1225187 | + | 477 | WP_005011624.1 | RadC family protein | - |
| BS654_RS06640 (1225250) | 1225250..1225471 | + | 222 | WP_001220314.1 | DUF987 domain-containing protein | - |
| BS654_RS06650 (1225634) | 1225634..1226008 | + | 375 | WP_001280958.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| BS654_RS06655 (1226055) | 1226055..1226429 | + | 375 | WP_000854917.1 | TA system toxin CbtA family protein | Toxin |
| BS654_RS06660 (1226426) | 1226426..1226917 | + | 492 | WP_000976828.1 | DUF5983 family protein | - |
| BS654_RS06665 (1226929) | 1226929..1227126 | + | 198 | WP_000839282.1 | DUF957 domain-containing protein | - |
| BS654_RS06670 (1227211) | 1227211..1227771 | + | 561 | Protein_1222 | DUF4942 domain-containing protein | - |
| BS654_RS06675 (1227843) | 1227843..1228847 | + | 1005 | WP_001179707.1 | IS110-like element ISSfl8 family transposase | - |
| BS654_RS06680 (1229129) | 1229129..1229410 | + | 282 | Protein_1224 | DUF4942 domain-containing protein | - |
| BS654_RS06690 (1229882) | 1229882..1230820 | + | 939 | Protein_1225 | glyoxylate/hydroxypyruvate reductase GhrA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 1227843..1228847 | 1004 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14145.22 Da Isoelectric Point: 7.7761
>T74263 WP_000854917.1 NZ_CP020336:1226055-1226429 [Shigella flexneri 4c]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK
Download Length: 375 bp
>T74263 NZ_CP020336:1226055-1226429 [Shigella flexneri 4c]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGTCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13793.62 Da Isoelectric Point: 6.4651
>AT74263 WP_001280958.1 NZ_CP020336:1225634-1226008 [Shigella flexneri 4c]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT74263 NZ_CP020336:1225634-1226008 [Shigella flexneri 4c]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | Q93EZ7 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0K4PAV2 |