Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5191150..5191703 Replicon chromosome
Accession NZ_CP020335
Organism Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0A0CTB7
Locus tag B0X70_RS22250 Protein ID WP_036810272.1
Coordinates 5191389..5191703 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1B8YBS3
Locus tag B0X70_RS22245 Protein ID WP_036778251.1
Coordinates 5191150..5191386 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0X70_RS22230 (B0X70_22405) 5186227..5187183 + 957 WP_217470420.1 HTH-type transcriptional regulator MetR -
B0X70_RS22235 (B0X70_22410) 5187389..5189038 - 1650 WP_217470421.1 Na+/H+ antiporter -
B0X70_RS22240 (B0X70_22415) 5189312..5190658 - 1347 WP_217471793.1 NCS2 family permease -
B0X70_RS22245 (B0X70_22420) 5191150..5191386 + 237 WP_036778251.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
B0X70_RS22250 (B0X70_22425) 5191389..5191703 + 315 WP_036810272.1 CcdB family protein Toxin
B0X70_RS22255 (B0X70_22430) 5191754..5192941 - 1188 WP_217470422.1 MFS transporter -
B0X70_RS22260 (B0X70_22435) 5193050..5193802 - 753 WP_217470423.1 sulfite exporter TauE/SafE family protein -
B0X70_RS22265 (B0X70_22440) 5194498..5195628 + 1131 WP_046397199.1 ISAs1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 5194498..5195628 1130


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(9-78)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11751.73 Da        Isoelectric Point: 7.1619

>T74258 WP_036810272.1 NZ_CP020335:5191389-5191703 [Photorhabdus akhurstii]
MQFTVYRNTGKTVVYPLLLDVTSDIIGQLNRRIVIPLLPIEKYPGSSRPVRMIPLIRLIDDKEYAVMTHELASISVHALG
AEFCDASQYRNQVKAALDFLFDGI

Download         Length: 315 bp

>T74258 NZ_CP020335:5191389-5191703 [Photorhabdus akhurstii]
ATGCAATTCACCGTTTATCGTAATACTGGGAAAACTGTTGTTTATCCCCTGTTGCTGGACGTCACGAGTGACATTATCGG
GCAGTTAAACCGCCGAATTGTGATCCCACTGCTGCCAATTGAAAAATATCCTGGCAGCAGTCGCCCTGTTCGAATGATCC
CCCTTATCAGGCTGATAGACGATAAAGAATATGCTGTCATGACTCACGAACTAGCCAGTATATCTGTTCATGCTTTGGGT
GCTGAATTTTGCGATGCATCGCAATACCGTAATCAGGTAAAAGCGGCACTCGATTTCCTCTTTGACGGTATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 9096.08 Da        Isoelectric Point: 6.2207

>AT74258 WP_036778251.1 NZ_CP020335:5191150-5191386 [Photorhabdus akhurstii]
MTARQRNTQSVTMTVERGLLNRAREAGINFSATLTAALDAELRHREALKWQEDNREALEVLNRFHDEHGCFSDEYRTF

Download         Length: 237 bp

>AT74258 NZ_CP020335:5191150-5191386 [Photorhabdus akhurstii]
ATGACAGCAAGACAACGCAACACACAGAGCGTCACAATGACTGTAGAACGAGGCTTACTGAACCGGGCACGTGAAGCAGG
TATTAACTTTAGTGCCACACTGACCGCCGCCTTGGATGCCGAATTACGCCATCGCGAGGCGCTGAAATGGCAGGAAGATA
ATAGGGAAGCTCTGGAGGTATTAAACCGTTTCCATGACGAACATGGTTGTTTCAGTGATGAATACAGGACATTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0CTB7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8YBS3

References