Detailed information of TA system TA074257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4649384..4650021
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2S8R2Y9
• Locus tag: B0X70_RS20125
• Protein ID: WP_071647067.1
• Coordinates: 4649899..4650021 (-)
• Length: 41 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1B8YHV7
• Locus tag: B0X70_RS20120
• Protein ID: WP_036808644.1
• Coordinates: 4649384..4649794 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS20090 (B0X70_20300)	4645452..4646078	+	627	WP_217470236.1	tail fiber assembly protein	-
B0X70_RS25400	4646687..4647043	-	357	Protein_3942	Bro-N domain-containing protein	-
B0X70_RS20100 (B0X70_20310)	4647425..4647748	+	324	WP_217470238.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B0X70_RS20105 (B0X70_20315)	4647795..4648091	+	297	WP_113044380.1	NadS family protein	-
B0X70_RS20110	4648515..4648736	+	222	WP_217470239.1	hypothetical protein	-
B0X70_RS20115 (B0X70_20325)	4648746..4649015	+	270	WP_065390442.1	helix-turn-helix transcriptional regulator	-
B0X70_RS20120 (B0X70_20330)	4649384..4649794	-	411	WP_036808644.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B0X70_RS20125 (B0X70_20335)	4649899..4650021	-	123	WP_071647067.1	mRNA interferase	Toxin
B0X70_RS20130 (B0X70_20340)	4650141..4650727	-	587	Protein_3949	hypothetical protein	-
B0X70_RS20135	4651418..4651582	-	165	WP_162473447.1	hypothetical protein	-
B0X70_RS20140 (B0X70_20345)	4651818..4652762	-	945	WP_036808642.1	class I SAM-dependent methyltransferase	-
B0X70_RS20145 (B0X70_20350)	4652900..4653433	-	534	WP_217470240.1	isopentenyl-diphosphate Delta-isomerase	-
B0X70_RS20150 (B0X70_20355)	4653997..4654932	+	936	WP_036808636.1	VirK/YbjX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4597780..4650730	52950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4721.38 Da
• Isoelectric Point: 11.5414

>T74257 WP_071647067.1 NZ_CP020335:c4650021-4649899 [Photorhabdus akhurstii]
VKQSEFRRWLEAQGVEVSNGTNHLKLRYQGKRSIMPRHPS

Nucleotide

• Length: 123 bp

>T74257 NZ_CP020335:c4650021-4649899 [Photorhabdus akhurstii]
GTGAAGCAAAGCGAGTTTAGACGGTGGCTGGAAGCTCAAGGCGTCGAAGTTTCAAATGGGACAAACCATCTGAAACTGAG
ATATCAGGGGAAACGAAGCATTATGCCGAGACACCCTAGCTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15131.32 Da
• Isoelectric Point: 4.2783

>AT74257 WP_036808644.1 NZ_CP020335:c4649794-4649384 [Photorhabdus akhurstii]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGDTREEALEMALDALITAFEFYFEDNEKIPLPGPVGQDYVDVPLSVASKVLM
LNAFLDSKLTQIELANRMGVKKQEVTRIFDLRHSTKIDTVGKVASAIGHQLTLSIE

Nucleotide

• Length: 411 bp

>AT74257 NZ_CP020335:c4649794-4649384 [Photorhabdus akhurstii]
ATGCGATATCCAGTCACACTAGAGCCAGTAGAAGAGGGCGGTTATTTCGTCTCGTTCCCGGATATCCCCGAAGCTTTAAC
CCAGGGGGATACACGGGAAGAGGCGCTAGAGATGGCACTCGATGCGCTAATAACCGCCTTTGAATTTTACTTCGAAGATA
ATGAAAAGATCCCGCTTCCTGGCCCCGTAGGGCAGGATTATGTTGATGTGCCCTTAAGTGTGGCCTCTAAGGTGCTTATG
CTCAATGCGTTTCTTGATTCCAAATTAACGCAAATTGAATTAGCCAACCGTATGGGGGTGAAAAAGCAGGAAGTGACCCG
TATCTTTGATTTACGGCACTCAACTAAAATTGATACGGTGGGTAAGGTCGCATCGGCTATCGGACATCAGTTAACTTTGT
CCATTGAATGA

Structures

Toxin

• AlphaFold DB: A0A2S8R2Y9

Antitoxin

• AlphaFold DB: A0A1B8YHV7