Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4649384..4650021 Replicon chromosome
Accession NZ_CP020335
Organism Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)


Gene name hicA Uniprot ID A0A2S8R2Y9
Locus tag B0X70_RS20125 Protein ID WP_071647067.1
Coordinates 4649899..4650021 (-) Length 41 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1B8YHV7
Locus tag B0X70_RS20120 Protein ID WP_036808644.1
Coordinates 4649384..4649794 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0X70_RS20090 (B0X70_20300) 4645452..4646078 + 627 WP_217470236.1 tail fiber assembly protein -
B0X70_RS25400 4646687..4647043 - 357 Protein_3942 Bro-N domain-containing protein -
B0X70_RS20100 (B0X70_20310) 4647425..4647748 + 324 WP_217470238.1 type II toxin-antitoxin system RelE/ParE family toxin -
B0X70_RS20105 (B0X70_20315) 4647795..4648091 + 297 WP_113044380.1 NadS family protein -
B0X70_RS20110 4648515..4648736 + 222 WP_217470239.1 hypothetical protein -
B0X70_RS20115 (B0X70_20325) 4648746..4649015 + 270 WP_065390442.1 helix-turn-helix transcriptional regulator -
B0X70_RS20120 (B0X70_20330) 4649384..4649794 - 411 WP_036808644.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B0X70_RS20125 (B0X70_20335) 4649899..4650021 - 123 WP_071647067.1 mRNA interferase Toxin
B0X70_RS20130 (B0X70_20340) 4650141..4650727 - 587 Protein_3949 hypothetical protein -
B0X70_RS20135 4651418..4651582 - 165 WP_162473447.1 hypothetical protein -
B0X70_RS20140 (B0X70_20345) 4651818..4652762 - 945 WP_036808642.1 class I SAM-dependent methyltransferase -
B0X70_RS20145 (B0X70_20350) 4652900..4653433 - 534 WP_217470240.1 isopentenyl-diphosphate Delta-isomerase -
B0X70_RS20150 (B0X70_20355) 4653997..4654932 + 936 WP_036808636.1 VirK/YbjX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4597780..4650730 52950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-65)


Sequences

Toxin        


Download         Length: 41 a.a.        Molecular weight: 4721.38 Da        Isoelectric Point: 11.5414

>T74257 WP_071647067.1 NZ_CP020335:c4650021-4649899 [Photorhabdus akhurstii]
VKQSEFRRWLEAQGVEVSNGTNHLKLRYQGKRSIMPRHPS

Download         Length: 123 bp

>T74257 NZ_CP020335:c4650021-4649899 [Photorhabdus akhurstii]
GTGAAGCAAAGCGAGTTTAGACGGTGGCTGGAAGCTCAAGGCGTCGAAGTTTCAAATGGGACAAACCATCTGAAACTGAG
ATATCAGGGGAAACGAAGCATTATGCCGAGACACCCTAGCTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15131.32 Da        Isoelectric Point: 4.2783

>AT74257 WP_036808644.1 NZ_CP020335:c4649794-4649384 [Photorhabdus akhurstii]
MRYPVTLEPVEEGGYFVSFPDIPEALTQGDTREEALEMALDALITAFEFYFEDNEKIPLPGPVGQDYVDVPLSVASKVLM
LNAFLDSKLTQIELANRMGVKKQEVTRIFDLRHSTKIDTVGKVASAIGHQLTLSIE

Download         Length: 411 bp

>AT74257 NZ_CP020335:c4649794-4649384 [Photorhabdus akhurstii]
ATGCGATATCCAGTCACACTAGAGCCAGTAGAAGAGGGCGGTTATTTCGTCTCGTTCCCGGATATCCCCGAAGCTTTAAC
CCAGGGGGATACACGGGAAGAGGCGCTAGAGATGGCACTCGATGCGCTAATAACCGCCTTTGAATTTTACTTCGAAGATA
ATGAAAAGATCCCGCTTCCTGGCCCCGTAGGGCAGGATTATGTTGATGTGCCCTTAAGTGTGGCCTCTAAGGTGCTTATG
CTCAATGCGTTTCTTGATTCCAAATTAACGCAAATTGAATTAGCCAACCGTATGGGGGTGAAAAAGCAGGAAGTGACCCG
TATCTTTGATTTACGGCACTCAACTAAAATTGATACGGTGGGTAAGGTCGCATCGGCTATCGGACATCAGTTAACTTTGT
CCATTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2S8R2Y9

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8YHV7

References