Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) VapXD/-
Location 4579448..4579921 Replicon chromosome
Accession NZ_CP020335
Organism Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)


Gene name VapD Uniprot ID A0A0A0CS68
Locus tag B0X70_RS19625 Protein ID WP_036808672.1
Coordinates 4579448..4579729 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name VapX Uniprot ID A0A0A0CUB4
Locus tag B0X70_RS19630 Protein ID WP_036808671.1
Coordinates 4579730..4579921 (-) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0X70_RS19600 (B0X70_19795) 4575274..4575675 - 402 WP_217470175.1 DUF2628 domain-containing protein -
B0X70_RS19605 (B0X70_19800) 4576102..4576809 + 708 WP_217470176.1 DUF1190 family protein -
B0X70_RS19610 (B0X70_19805) 4576816..4577976 + 1161 WP_217470177.1 glutathionylspermidine synthase family protein -
B0X70_RS19615 (B0X70_19810) 4578086..4578565 + 480 WP_036808674.1 Hcp family type VI secretion system effector -
B0X70_RS19620 (B0X70_19815) 4578699..4579142 - 444 WP_217470178.1 hypothetical protein -
B0X70_RS19625 (B0X70_19820) 4579448..4579729 - 282 WP_036808672.1 virulence factor Toxin
B0X70_RS19630 (B0X70_19825) 4579730..4579921 - 192 WP_036808671.1 DUF5397 family protein Antitoxin
B0X70_RS19635 (B0X70_19830) 4580328..4581611 - 1284 WP_217470179.1 DUF1835 domain-containing protein -
B0X70_RS19640 (B0X70_19835) 4581801..4582586 - 786 WP_110091026.1 4,5-DOPA dioxygenase extradiol -
B0X70_RS19645 (B0X70_19840) 4582781..4583434 - 654 WP_110091027.1 3,4-dihydroxy-2-butanone-4-phosphate synthase -
B0X70_RS19650 (B0X70_19845) 4583902..4584189 + 288 WP_040148857.1 accessory factor UbiK family protein -
B0X70_RS19655 (B0X70_19850) 4584258..4584920 - 663 WP_036808664.1 DUF799 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Virulence-associated protein D / CRISPR associated protein Cas2

(1-86)

Antitoxin

Protein of unknown function DUF5397

(2-62)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10617.03 Da        Isoelectric Point: 5.6306

>T74255 WP_036808672.1 NZ_CP020335:c4579729-4579448 [Photorhabdus akhurstii]
MYAIAFDLVVVDTEQHHPKGVSQAYAEIGATLAQYGFRRVQGSLYISDDENMAQLFMAIQSLKAQSWFPPSVRDIRAFRI
EQWSDFTSVVKDK

Download         Length: 282 bp

>T74255 NZ_CP020335:c4579729-4579448 [Photorhabdus akhurstii]
ATGTACGCCATTGCATTTGATTTAGTTGTTGTTGACACAGAACAGCATCACCCTAAAGGGGTCTCACAAGCGTACGCTGA
AATTGGTGCAACGCTGGCGCAATATGGTTTTCGGCGTGTACAAGGCAGTTTGTATATCAGTGACGATGAGAATATGGCTC
AGCTTTTTATGGCGATCCAATCCCTCAAGGCGCAATCTTGGTTTCCTCCGTCAGTCCGTGATATTCGTGCGTTCCGTATT
GAACAGTGGTCTGATTTTACGTCCGTAGTCAAAGATAAGTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 6996.05 Da        Isoelectric Point: 4.9861

>AT74255 WP_036808671.1 NZ_CP020335:c4579921-4579730 [Photorhabdus akhurstii]
MISVPRVPVGTIKSFGAFGPKYEVGQLIRPLEDGDWLVEVVLVETGEKTEYRLKHVNDDPKAA

Download         Length: 192 bp

>AT74255 NZ_CP020335:c4579921-4579730 [Photorhabdus akhurstii]
ATGATAAGTGTTCCCCGAGTACCTGTCGGTACAATTAAAAGCTTTGGTGCATTTGGGCCTAAATATGAAGTGGGGCAACT
GATTCGCCCGTTAGAGGATGGTGACTGGTTGGTTGAGGTTGTGCTGGTTGAAACTGGTGAGAAGACTGAATATCGTCTGA
AACATGTCAACGATGATCCTAAGGCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0CS68

Antitoxin

Source ID Structure
AlphaFold DB A0A0A0CUB4

References