Detailed information of TA system TA074250

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2880669..2881320
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A4R4JBH0
• Locus tag: B0X70_RS12565
• Protein ID: WP_036805646.1
• Coordinates: 2881141..2881320 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B0X70_RS12560
• Protein ID: WP_110087358.1
• Coordinates: 2880669..2881082 (-)
• Length: 138 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS12540 (B0X70_12650)	2876591..2877553	-	963	WP_044466996.1	UTP--glucose-1-phosphate uridylyltransferase GalU	-
B0X70_RS12545 (B0X70_12655)	2877934..2878959	-	1026	WP_036777158.1	two-component system response regulator RssB	-
B0X70_RS12550 (B0X70_12660)	2879118..2879597	+	480	WP_217469478.1	YchJ family protein	-
B0X70_RS12555 (B0X70_12665)	2879643..2880491	+	849	WP_217469479.1	formyltetrahydrofolate deformylase	-
B0X70_RS12560 (B0X70_12670)	2880669..2881082	-	414	WP_110087358.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B0X70_RS12565 (B0X70_12675)	2881141..2881320	-	180	WP_036805646.1	type II toxin-antitoxin system HicA family toxin	Toxin
B0X70_RS12595 (B0X70_12705)	2883041..2883946	+	906	WP_217469480.1	NAD(P)-dependent oxidoreductase	-
B0X70_RS12600 (B0X70_12710)	2883946..2885223	+	1278	WP_217469481.1	four-carbon acid sugar kinase family protein	-
B0X70_RS12605 (B0X70_12715)	2885216..2885854	+	639	WP_046397687.1	aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6897.16 Da
• Isoelectric Point: 10.9941

>T74250 WP_036805646.1 NZ_CP020335:c2881320-2881141 [Photorhabdus akhurstii]
MRSADLIKEVEKKGWVLVRINGSHHHFKHPERNDLLTIPHPKQEIKVGLLRKIQKQVGL

Nucleotide

• Length: 180 bp

>T74250 NZ_CP020335:c2881320-2881141 [Photorhabdus akhurstii]
TTGAGAAGTGCAGATTTAATCAAAGAAGTTGAGAAAAAAGGGTGGGTACTGGTAAGAATCAACGGGAGTCATCATCATTT
TAAACACCCTGAACGTAATGACTTGTTGACAATTCCACATCCCAAACAGGAAATCAAAGTGGGATTGCTTAGGAAAATAC
AGAAGCAAGTGGGGCTGTAA

Antitoxin

Protein

• Length: 138 a.a.
• Molecular weight: 15225.24 Da
• Isoelectric Point: 4.3465

>AT74250 WP_110087358.1 NZ_CP020335:c2881082-2880669 [Photorhabdus akhurstii]
VLYPIAIEIGDENHAYGVVVPDISGCFSAGDSMEDVIKNAKEAISGHLELLAEMNQLPPEAKPLDYWIKDEEYSGWAWAV
IDVDVEPYLGKSTKFNVTLPDLLSKKIDDQVKASPNLYKNRSHFLQVAALHELQNGI

Nucleotide

• Length: 414 bp

>AT74250 NZ_CP020335:c2881082-2880669 [Photorhabdus akhurstii]
GTGTTATATCCTATTGCGATTGAAATTGGTGATGAGAACCACGCTTACGGCGTGGTTGTGCCCGATATATCAGGTTGTTT
CTCGGCAGGTGATTCAATGGAGGATGTAATAAAAAATGCCAAGGAAGCTATCTCTGGGCATTTAGAGTTATTGGCCGAAA
TGAACCAATTACCCCCTGAAGCAAAGCCATTAGATTACTGGATCAAAGATGAAGAATACTCAGGGTGGGCATGGGCTGTA
ATTGACGTTGATGTTGAACCATATTTGGGTAAATCGACAAAATTCAACGTCACTCTGCCTGATTTGTTATCAAAGAAAAT
CGATGATCAAGTTAAAGCAAGTCCAAACCTGTATAAGAATCGTAGCCACTTTTTGCAAGTGGCTGCTTTGCATGAATTGC
AAAACGGTATTTAG

Structures

Toxin

• AlphaFold DB: A0A4R4JBH0