Detailed information of TA system TA074249

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2751182..2751759
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2S8PUW8
• Locus tag: B0X70_RS12065
• Protein ID: WP_040148713.1
• Coordinates: 2751427..2751759 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B0X70_RS12060
• Protein ID: WP_217471589.1
• Coordinates: 2751182..2751427 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS12035 (B0X70_12150)	2747759..2748760	+	1002	WP_040148717.1	adenosine deaminase	-
B0X70_RS12040 (B0X70_12155)	2748978..2749214	+	237	WP_040148716.1	hypothetical protein	-
B0X70_RS12050	2749526..2749879	-	354	WP_217471815.1	hypothetical protein	-
B0X70_RS12055 (B0X70_12165)	2749881..2750738	-	858	WP_217471588.1	glycosyltransferase	-
B0X70_RS12060 (B0X70_12170)	2751182..2751427	+	246	WP_217471589.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B0X70_RS12065 (B0X70_12175)	2751427..2751759	+	333	WP_040148713.1	endoribonuclease MazF	Toxin
B0X70_RS12070 (B0X70_12180)	2751972..2752943	-	972	WP_217471590.1	bile acid:sodium symporter family protein	-
B0X70_RS12075 (B0X70_12185)	2753028..2754065	-	1038	WP_217471591.1	oxidoreductase	-
B0X70_RS12080 (B0X70_12190)	2754262..2754702	+	441	WP_036805863.1	DUF2569 domain-containing protein	-
B0X70_RS12085 (B0X70_12195)	2754825..2755406	+	582	WP_011146612.1	electron transport complex subunit RsxA	-
B0X70_RS12090 (B0X70_12200)	2755406..2756038	+	633	WP_036805859.1	electron transport complex subunit RsxB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12001.73 Da
• Isoelectric Point: 7.1061

>T74249 WP_040148713.1 NZ_CP020335:2751427-2751759 [Photorhabdus akhurstii]
MVSRFVPDAGDIIWIDFDPVTGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKAKDYPFEVELSGSRDSVALADQVTCV
DWRARKVTKKGNVNSSELEDIRAKAKALIG

Nucleotide

• Length: 333 bp

>T74249 NZ_CP020335:2751427-2751759 [Photorhabdus akhurstii]
ATGGTTTCTCGTTTTGTGCCTGATGCTGGCGATATTATCTGGATTGACTTTGATCCTGTGACTGGACACGAACAGGGAGG
CCATCGCCCCGCAGTAGTACTGAGTCCATTTGCATATAACAATAAAGTGGGGTTACTACTCTGTGTTCCTTGCACAACGA
AAGCAAAAGATTATCCTTTTGAAGTTGAACTATCAGGAAGCCGCGATAGTGTCGCCTTAGCTGACCAGGTAACATGCGTT
GATTGGCGAGCCAGAAAAGTGACAAAAAAAGGCAATGTTAATTCTTCAGAACTGGAAGATATAAGAGCTAAAGCAAAAGC
GCTTATCGGATAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 5.0022

>AT74249 WP_217471589.1 NZ_CP020335:2751182-2751427 [Photorhabdus akhurstii]
MARVVVKKWGNSPSVRLPVAIMQAASLNVDDTVDITVEDGRIIIIPVRAKEYSPDALLAGITPENIHEKIDFGSPVGKEL
I

Nucleotide

• Length: 246 bp

>AT74249 NZ_CP020335:2751182-2751427 [Photorhabdus akhurstii]
ATGGCACGAGTAGTAGTGAAAAAATGGGGTAACAGCCCTTCTGTACGTCTTCCGGTAGCTATCATGCAAGCCGCCTCACT
TAATGTCGATGATACTGTTGATATTACTGTCGAAGATGGTCGAATTATCATTATTCCAGTCAGGGCGAAAGAGTATTCTC
CAGATGCGCTATTAGCAGGAATTACACCGGAAAATATTCATGAAAAAATAGATTTTGGTTCTCCCGTGGGTAAGGAATTG
ATCTGA

Structures

Toxin

• AlphaFold DB: A0A2S8PUW8