Detailed information of TA system TA074246

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2587995..2588605
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: B0X70_RS11415
• Protein ID: WP_040150720.1
• Coordinates: 2588213..2588605 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A022PC85
• Locus tag: B0X70_RS11410
• Protein ID: WP_036780625.1
• Coordinates: 2587995..2588216 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS11380 (B0X70_11495)	2583625..2583897	-	273	WP_036775719.1	DUF1161 domain-containing protein	-
B0X70_RS11385 (B0X70_11500)	2583920..2583988	-	69	Protein_2198	DUF1283 domain-containing protein	-
B0X70_RS25160 (B0X70_11505)	2584099..2584827	-	729	Protein_2199	hypothetical protein	-
B0X70_RS11390 (B0X70_11510)	2584943..2585524	-	582	WP_217471520.1	carbonic anhydrase	-
B0X70_RS11395 (B0X70_11515)	2585774..2586268	+	495	WP_246593495.1	cupin domain-containing protein	-
B0X70_RS11400 (B0X70_11520)	2586539..2586775	+	237	WP_217471521.1	type II toxin-antitoxin system ParD family antitoxin	-
B0X70_RS11405 (B0X70_11525)	2586950..2587543	+	594	Protein_2203	DJ-1/PfpI family protein	-
B0X70_RS11410 (B0X70_11530)	2587995..2588216	+	222	WP_036780625.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
B0X70_RS11415 (B0X70_11535)	2588213..2588605	+	393	WP_040150720.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
B0X70_RS11420 (B0X70_11540)	2589072..2591027	+	1956	WP_217471522.1	PTS mannitol transporter subunit IICBA	-
B0X70_RS11425 (B0X70_11545)	2591043..2592203	+	1161	WP_217471523.1	mannitol-1-phosphate 5-dehydrogenase	-
B0X70_RS11430 (B0X70_11550)	2592294..2592836	+	543	WP_217471524.1	MltR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14727.78 Da
• Isoelectric Point: 8.9081

>T74246 WP_040150720.1 NZ_CP020335:2588213-2588605 [Photorhabdus akhurstii]
MIWVSAQEVIAFHDRILRRFPGVAGIADPGRAQALIYRVQNRVHYEGVTDLFELAATYWVAIARGHIFNDGNKRTAFLVT
MSFLWRNGICIRNNGNTLENLTVEAATGEKTVSQLAQYLRELVDSPNSRY

Nucleotide

• Length: 393 bp

>T74246 NZ_CP020335:2588213-2588605 [Photorhabdus akhurstii]
ATGATTTGGGTGAGTGCTCAAGAAGTTATTGCCTTCCACGATCGCATACTTCGACGTTTTCCCGGCGTTGCCGGGATAGC
AGATCCTGGTAGAGCGCAGGCATTGATTTATCGTGTGCAAAATCGGGTTCACTATGAAGGCGTTACTGACCTTTTTGAAC
TCGCCGCAACTTACTGGGTAGCGATTGCTCGTGGTCATATCTTTAATGACGGTAATAAGCGGACCGCATTTCTCGTGACC
ATGAGCTTTCTTTGGCGTAATGGTATTTGCATTCGCAATAACGGCAATACGCTGGAAAACTTAACTGTAGAAGCTGCTAC
TGGTGAGAAAACTGTCAGTCAGTTGGCTCAATATTTGCGGGAACTAGTGGACAGTCCGAATTCACGTTATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7997.93 Da
• Isoelectric Point: 4.9556

>AT74246 WP_036780625.1 NZ_CP020335:2587995-2588216 [Photorhabdus akhurstii]
MQTYTSTQARANISVVLDTAVNGEPVEITRRDGSAAVVISKAEFEVYQNAKLDAEFDAMMQRHGHTVAALTDR

Nucleotide

• Length: 222 bp

>AT74246 NZ_CP020335:2587995-2588216 [Photorhabdus akhurstii]
ATGCAAACCTATACCTCAACTCAGGCCAGAGCCAATATTTCTGTGGTGCTGGATACCGCAGTCAATGGTGAACCTGTTGA
AATCACCCGTCGGGACGGCAGCGCTGCCGTAGTGATCAGTAAAGCGGAATTCGAGGTGTATCAGAATGCAAAGCTGGATG
CCGAGTTTGATGCCATGATGCAACGTCATGGTCATACAGTAGCAGCGTTGACTGACCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A022PC85