Detailed information of TA system TA074242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2376051..2376688
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A2S8QI15
• Locus tag: B0X70_RS10420
• Protein ID: WP_040152635.1
• Coordinates: 2376281..2376688 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: B0X70_RS10415
• Protein ID: WP_110090524.1
• Coordinates: 2376051..2376281 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS10395 (B0X70_10500)	2371856..2372014	-	159	WP_071647063.1	DUF2474 domain-containing protein	-
B0X70_RS10400 (B0X70_10505)	2371995..2373002	-	1008	WP_040152628.1	cytochrome d ubiquinol oxidase subunit II	-
B0X70_RS10405 (B0X70_10510)	2372992..2374329	-	1338	WP_040152629.1	cytochrome ubiquinol oxidase subunit I	-
B0X70_RS10410 (B0X70_10515)	2374461..2375888	+	1428	WP_217471456.1	PLP-dependent aminotransferase family protein	-
B0X70_RS10415 (B0X70_10520)	2376051..2376281	+	231	WP_110090524.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0X70_RS10420 (B0X70_10525)	2376281..2376688	+	408	WP_040152635.1	tRNA(fMet)-specific endonuclease VapC	Toxin
B0X70_RS10425 (B0X70_10530)	2376773..2377615	-	843	WP_040152636.1	hypothetical protein	-
B0X70_RS10430 (B0X70_10535)	2378034..2378840	-	807	WP_217471457.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15264.68 Da
• Isoelectric Point: 7.8033

>T74242 WP_040152635.1 NZ_CP020335:2376281-2376688 [Photorhabdus akhurstii]
MLKYMLDTCICIYTLKNQPQRVREAFLLHHDQLCISTVTLMELIKGAEKSGNPESNMKIIEGFVARLRVLDFDSKAAVHA
GQIIAELERGGLRIGSYDNQIAGHARSQGLIIVTNNVREFERVPGLRVENWVNEK

Nucleotide

• Length: 408 bp

>T74242 NZ_CP020335:2376281-2376688 [Photorhabdus akhurstii]
ATGCTGAAATACATGCTTGATACCTGTATTTGCATCTACACTCTTAAGAACCAGCCCCAGAGAGTGCGCGAAGCTTTTCT
ACTCCATCACGATCAGTTGTGTATTAGCACGGTGACATTGATGGAGTTGATTAAAGGCGCAGAGAAATCGGGAAATCCAG
AAAGCAATATGAAGATTATTGAGGGATTCGTTGCTAGGTTGAGGGTATTGGATTTCGACAGTAAGGCTGCTGTGCATGCC
GGTCAGATTATCGCAGAGCTTGAACGGGGCGGTTTACGCATAGGGTCTTATGACAATCAAATAGCGGGGCATGCCAGAAG
CCAAGGGTTGATTATCGTGACTAATAATGTGCGCGAATTTGAGCGGGTTCCTGGCCTTCGTGTTGAGAACTGGGTCAACG
AAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8533.60 Da
• Isoelectric Point: 4.6855

>AT74242 WP_110090524.1 NZ_CP020335:2376051-2376281 [Photorhabdus akhurstii]
MVQTTVFKSNRSQAVRLPKAVAFPEDVKWVDVVAVGHTRIITPAGESWDSWFDGPSVTADFMAEREQPPVQEREGF

Nucleotide

• Length: 231 bp

>AT74242 NZ_CP020335:2376051-2376281 [Photorhabdus akhurstii]
ATGGTTCAAACAACGGTATTTAAAAGCAATCGTTCTCAGGCAGTCCGGCTTCCTAAAGCTGTCGCTTTTCCGGAAGATGT
GAAGTGGGTTGATGTTGTCGCTGTAGGCCATACTCGCATAATCACTCCGGCTGGTGAATCTTGGGACAGTTGGTTTGATG
GCCCTAGTGTTACGGCCGATTTTATGGCAGAACGCGAGCAGCCGCCAGTACAAGAGCGAGAGGGATTCTGA

Structures

Toxin

• AlphaFold DB: A0A2S8QI15