Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1727776..1728435 Replicon chromosome
Accession NZ_CP020335
Organism Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)


Gene name hicA Uniprot ID A0A1B8YH89
Locus tag B0X70_RS07525 Protein ID WP_046397782.1
Coordinates 1728247..1728435 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A329X5Y1
Locus tag B0X70_RS07520 Protein ID WP_036812864.1
Coordinates 1727776..1728183 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0X70_RS07500 (B0X70_07575) 1725448..1725765 + 318 WP_036812860.1 hypothetical protein -
B0X70_RS07505 (B0X70_07580) 1726007..1726270 - 264 Protein_1431 transcriptional regulator -
B0X70_RS07510 (B0X70_07585) 1726629..1727585 + 957 WP_217471208.1 tail fiber protein -
B0X70_RS07515 (B0X70_07590) 1727634..1727723 + 90 WP_217471209.1 tail fiber assembly protein -
B0X70_RS07520 (B0X70_07595) 1727776..1728183 - 408 WP_036812864.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
B0X70_RS07525 (B0X70_07600) 1728247..1728435 - 189 WP_046397782.1 type II toxin-antitoxin system HicA family toxin Toxin
B0X70_RS07530 (B0X70_07605) 1729143..1730045 + 903 WP_217471210.1 tail fiber protein -
B0X70_RS07535 (B0X70_07610) 1730384..1730740 + 357 WP_046396219.1 DUF2778 domain-containing protein -
B0X70_RS07540 (B0X70_07615) 1730737..1731120 + 384 WP_036812871.1 hypothetical protein -
B0X70_RS07545 (B0X70_07620) 1731530..1732402 + 873 WP_217471211.1 phage tail protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1726004..1789638 63634


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-53)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 6670.91 Da        Isoelectric Point: 11.1589

>T74240 WP_046397782.1 NZ_CP020335:c1728435-1728247 [Photorhabdus akhurstii]
MGSAELIKLLIADGWVKARQDGSHITLTKPGVAKIITIPHPRKDSSKGVIRQAQNISGLKLL

Download         Length: 189 bp

>T74240 NZ_CP020335:c1728435-1728247 [Photorhabdus akhurstii]
ATGGGTAGTGCAGAGCTAATAAAACTCCTAATAGCTGACGGATGGGTTAAGGCCAGACAAGACGGTAGTCACATCACTCT
AACTAAACCCGGTGTTGCAAAAATAATCACAATACCGCACCCAAGAAAAGACTCATCAAAAGGTGTTATCCGGCAGGCAC
AAAATATTTCAGGGCTGAAATTACTATGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 15164.14 Da        Isoelectric Point: 4.6719

>AT74240 WP_036812864.1 NZ_CP020335:c1728183-1727776 [Photorhabdus akhurstii]
MIYPLFIFKTEDGFDGYFPDVESCFFAGNTLEDAIRDAEKAFAQHMEVLIEHGGYVPAPKNSSDYIGDPRLSEDGGVIAL
IELDPAKYESKAIKFNLTMPGNLLTAIDRYIEKNGHYKNRSAFLAELARKEIAHQ

Download         Length: 408 bp

>AT74240 NZ_CP020335:c1728183-1727776 [Photorhabdus akhurstii]
ATGATTTATCCACTCTTTATATTTAAAACAGAAGATGGGTTTGACGGTTACTTCCCCGATGTTGAAAGCTGTTTCTTTGC
TGGCAATACCTTAGAAGATGCTATTCGGGATGCTGAAAAAGCGTTCGCGCAGCACATGGAAGTTTTAATCGAACACGGCG
GCTATGTCCCCGCTCCTAAAAACTCTTCTGATTACATCGGTGATCCTCGCTTGTCTGAAGATGGAGGGGTTATTGCCCTC
ATCGAGTTAGATCCAGCGAAATATGAGTCAAAAGCCATAAAGTTCAATTTAACTATGCCTGGCAATCTATTAACTGCCAT
AGATCGCTACATTGAAAAAAACGGACACTACAAAAACCGCTCTGCTTTTCTAGCTGAGTTAGCCAGAAAAGAAATAGCTC
ACCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8YH89

Antitoxin

Source ID Structure
AlphaFold DB A0A329X5Y1

References