Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 1146004..1146640 Replicon chromosome
Accession NZ_CP020335
Organism Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)


Gene name vapC Uniprot ID A0A0A0CT37
Locus tag B0X70_RS04965 Protein ID WP_036808120.1
Coordinates 1146224..1146640 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1B8YKE4
Locus tag B0X70_RS04960 Protein ID WP_036808122.1
Coordinates 1146004..1146237 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0X70_RS04945 (B0X70_05020) 1141507..1142892 + 1386 WP_040153441.1 DASS family sodium-coupled anion symporter -
B0X70_RS04950 (B0X70_05025) 1142983..1144185 - 1203 WP_217471022.1 MFS transporter -
B0X70_RS04955 (B0X70_05030) 1144712..1145647 - 936 WP_110086576.1 2OG-Fe(II) oxygenase family protein -
B0X70_RS04960 (B0X70_05035) 1146004..1146237 + 234 WP_036808122.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
B0X70_RS04965 (B0X70_05040) 1146224..1146640 + 417 WP_036808120.1 PIN domain-containing protein Toxin
B0X70_RS04970 (B0X70_05045) 1146763..1147701 - 939 WP_217471023.1 2OG-Fe(II) oxygenase family protein -
B0X70_RS04975 (B0X70_05050) 1148413..1149777 - 1365 WP_040153437.1 murein transglycosylase D -
B0X70_RS04980 (B0X70_05055) 1149849..1150601 - 753 WP_217471024.1 hydroxyacylglutathione hydrolase -
B0X70_RS04985 (B0X70_05060) 1150644..1151360 + 717 WP_217471025.1 methyltransferase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1125654..1155849 30195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-120)

Antitoxin

SpoVT-AbrB domain

(5-42)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15429.89 Da        Isoelectric Point: 5.1717

>T74237 WP_036808120.1 NZ_CP020335:1146224-1146640 [Photorhabdus akhurstii]
MTGAKSCDFIDSNVVLYLLSQDVAKADSAEALLKANPVISVQVLNEVAHVCVRKLKMSWEEIAEFLDIVRSFCKIVPLTV
EVHDRARFIAERYHLSFYDSCIVAAAVIAGCRALYSEDMNHGQMLEDSLVIKNPFSYV

Download         Length: 417 bp

>T74237 NZ_CP020335:1146224-1146640 [Photorhabdus akhurstii]
ATGACAGGGGCTAAGTCTTGCGATTTCATCGACAGCAATGTGGTGCTGTACCTGCTGTCCCAAGACGTGGCCAAGGCCGA
TAGTGCCGAAGCGCTGCTGAAAGCGAATCCGGTCATTAGTGTACAGGTGCTCAACGAAGTGGCGCACGTGTGTGTGCGTA
AACTTAAGATGAGTTGGGAAGAGATAGCGGAGTTCCTCGATATTGTACGTAGTTTTTGCAAAATCGTGCCTCTTACGGTT
GAAGTTCATGACCGAGCTAGGTTTATTGCAGAGCGTTATCACTTGTCATTCTACGATTCTTGTATTGTGGCGGCTGCGGT
CATCGCAGGTTGTCGGGCGCTCTATAGTGAAGACATGAATCACGGGCAGATGTTGGAAGACAGCTTGGTTATTAAAAATC
CATTCTCATATGTCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8753.04 Da        Isoelectric Point: 6.3478

>AT74237 WP_036808122.1 NZ_CP020335:1146004-1146237 [Photorhabdus akhurstii]
MQVAKWGNSLAIRLPASLVEILELREGDDIEIVVDDPRTFAVRKKPGSEVLLERLRTFRGKLPADFKFSRDEANDRG

Download         Length: 234 bp

>AT74237 NZ_CP020335:1146004-1146237 [Photorhabdus akhurstii]
ATGCAAGTAGCAAAATGGGGTAACAGCCTTGCCATACGTTTGCCGGCTAGTTTAGTTGAAATTCTGGAATTGCGTGAAGG
TGATGATATTGAGATTGTAGTGGATGATCCGCGTACCTTCGCGGTACGCAAGAAGCCTGGATCTGAGGTGTTGTTGGAGC
GTCTTCGGACATTCCGCGGTAAGTTGCCGGCAGATTTCAAGTTCAGTAGAGATGAAGCGAATGACAGGGGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0CT37

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8YKE4

References