Detailed information of TA system TA074235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1033597..1034305
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B0X70_RS04435
• Protein ID: WP_217470972.1
• Coordinates: 1033916..1034305 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1B8YIX8
• Locus tag: B0X70_RS04430
• Protein ID: WP_065390143.1
• Coordinates: 1033597..1033926 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS04420 (B0X70_04485)	1032960..1033154	+	195	WP_217470971.1	type II toxin-antitoxin system HicB family antitoxin	-
B0X70_RS04425 (B0X70_04490)	1033205..1033516	-	312	WP_105396049.1	XRE family transcriptional regulator	-
B0X70_RS04430 (B0X70_04495)	1033597..1033926	-	330	WP_065390143.1	helix-turn-helix domain-containing protein	Antitoxin
B0X70_RS04435 (B0X70_04500)	1033916..1034305	-	390	WP_217470972.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0X70_RS24980	1034437..1034520	-	84	Protein_860	hypothetical protein	-
B0X70_RS04440 (B0X70_04505)	1034573..1035628	-	1056	WP_217470973.1	ferric ABC transporter ATP-binding protein	-
B0X70_RS04445 (B0X70_04510)	1035644..1037722	-	2079	WP_217470974.1	iron ABC transporter permease	-
B0X70_RS04450 (B0X70_04515)	1037860..1038891	-	1032	WP_036808303.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (3-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-93)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14386.83 Da
• Isoelectric Point: 10.1700

>T74235 WP_217470972.1 NZ_CP020335:c1034305-1033916 [Photorhabdus akhurstii]
VAIYLTKLFNKKAKKLKITTDTLLKAASEVMNGVFEANLGGGVIKKRVPLKGTGKSGGTRTIIFFKSGTNLFFFDGWSKS
EVSGKRAKEIEDDDLEGYKDVAAELLKYDDKKIKKMLELKLLVEVKDEH

Nucleotide

• Length: 390 bp

>T74235 NZ_CP020335:c1034305-1033916 [Photorhabdus akhurstii]
GTGGCTATTTACTTAACAAAACTTTTTAATAAAAAAGCCAAAAAACTAAAAATCACCACAGATACCTTACTTAAAGCGGC
GTCTGAGGTCATGAATGGTGTATTTGAAGCTAATTTAGGCGGTGGTGTTATCAAGAAGCGGGTTCCTTTAAAAGGCACGG
GTAAAAGTGGTGGCACACGAACTATCATCTTCTTTAAAAGTGGAACTAACCTGTTTTTTTTCGATGGCTGGTCAAAAAGT
GAAGTCAGCGGAAAAAGAGCTAAGGAAATCGAGGACGATGACTTGGAGGGTTACAAAGACGTTGCCGCAGAACTTCTAAA
GTATGATGATAAAAAAATAAAAAAAATGCTCGAACTAAAACTTTTGGTAGAGGTGAAAGATGAGCACTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12151.20 Da
• Isoelectric Point: 10.4829

>AT74235 WP_065390143.1 NZ_CP020335:c1033926-1033597 [Photorhabdus akhurstii]
MSTKLDEMLGFAKELEEAGISNGDLTKKLEARIQVRNLSKHIAKARPMSGDEIKKLRDRYNISQASLALALNMTKESVSK
WERGEIKPNGAALRMLNLIDKQGLKIITA

Nucleotide

• Length: 330 bp

>AT74235 NZ_CP020335:c1033926-1033597 [Photorhabdus akhurstii]
ATGAGCACTAAATTGGACGAAATGCTTGGCTTTGCAAAAGAGCTAGAAGAGGCTGGTATTTCTAACGGTGATTTAACCAA
AAAACTTGAAGCTAGAATTCAAGTTCGCAATTTAAGTAAACATATAGCTAAAGCTCGCCCGATGTCTGGCGATGAAATCA
AGAAATTACGCGACCGTTATAACATTTCTCAAGCTAGTTTAGCTCTGGCGCTTAATATGACGAAAGAAAGCGTATCAAAA
TGGGAGCGTGGTGAAATTAAGCCCAATGGGGCAGCATTAAGAATGCTAAACCTCATTGATAAACAAGGATTGAAAATTAT
CACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1B8YIX8