Detailed information of TA system TA074233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 718880..719550
• Replicon: chromosome
• Accession: NZ_CP020335
• Organism: Photorhabdus akhurstii strain 0813-124 phase II

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B0X70_RS03105
• Protein ID: WP_217470862.1
• Coordinates: 718880..719299 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A2S8QFB6
• Locus tag: B0X70_RS03110
• Protein ID: WP_040150445.1
• Coordinates: 719296..719550 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0X70_RS03100 (B0X70_03160)	716739..718784	+	2046	WP_217470861.1	hypothetical protein	-
B0X70_RS03105 (B0X70_03165)	718880..719299	-	420	WP_217470862.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0X70_RS03110 (B0X70_03170)	719296..719550	-	255	WP_040150445.1	Arc family DNA-binding protein	Antitoxin
B0X70_RS03115 (B0X70_03175)	721016..722029	-	1014	WP_217470863.1	IS630-like element ISPlu10 family transposase	-
B0X70_RS03120 (B0X70_03180)	722521..722985	+	465	WP_052105826.1	avidin/streptavidin family protein	-
B0X70_RS03125	723132..723284	+	153	Protein_617	IS4/IS5 family transposase	-
B0X70_RS03130 (B0X70_03185)	723716..724093	-	378	WP_217470865.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	721016..722029	1013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15129.49 Da
• Isoelectric Point: 4.8537

>T74233 WP_217470862.1 NZ_CP020335:c719299-718880 [Photorhabdus akhurstii]
MIILDTNVISEPLRQAPESRVIKWLDAQPIETLYLSAITVAELRFGLASLPIGKRRDKLQENLEKQVLPLFTGRVLSFDM
SASQSYSELMAKARIAGLAINVADGYIAATAAANGMIVATRDVSPFEAAGVNVINPWEN

Nucleotide

• Length: 420 bp

>T74233 NZ_CP020335:c719299-718880 [Photorhabdus akhurstii]
ATGATTATACTGGATACTAACGTGATTTCGGAACCACTGCGTCAGGCTCCTGAATCACGTGTCATTAAGTGGCTAGACGC
ACAACCGATTGAAACCCTATATCTGTCGGCAATAACCGTAGCCGAACTGCGCTTTGGTTTGGCTTCACTGCCCATAGGGA
AACGCCGCGATAAATTGCAGGAGAACCTGGAAAAGCAGGTATTACCCTTGTTCACCGGACGTGTATTATCATTCGACATG
TCAGCGTCACAATCTTATAGTGAACTTATGGCAAAAGCCCGGATCGCCGGTTTGGCAATCAACGTGGCCGATGGATATAT
CGCTGCAACTGCCGCCGCAAATGGCATGATAGTTGCCACACGAGATGTTAGCCCGTTCGAGGCAGCTGGTGTGAACGTGA
TCAATCCCTGGGAGAATTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9444.89 Da
• Isoelectric Point: 6.4735

>AT74233 WP_040150445.1 NZ_CP020335:c719550-719296 [Photorhabdus akhurstii]
MAMMTVRNIPDEVHRALRVRAAIHGRSAEAEVRAILEESVKAEGRIKLGSMLAEIGRQSKLTDEEFTAIEQIRDKIPANP
MNFE

Nucleotide

• Length: 255 bp

>AT74233 NZ_CP020335:c719550-719296 [Photorhabdus akhurstii]
ATGGCAATGATGACTGTCAGGAACATCCCTGATGAAGTACACCGTGCTTTACGAGTACGTGCTGCTATTCATGGCCGCAG
TGCTGAAGCCGAAGTACGCGCCATTCTGGAAGAATCCGTAAAAGCCGAAGGTCGGATCAAGTTAGGATCGATGCTAGCTG
AGATTGGCCGCCAATCCAAGTTAACCGATGAGGAGTTTACAGCCATTGAGCAAATACGTGACAAAATTCCTGCTAACCCG
ATGAATTTTGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2S8QFB6