Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 5169..5854 Replicon plasmid pMM170
Accession NZ_CP020333
Organism Martelella mediterranea DSM 17316 strain MACL11

Toxin (Protein)


Gene name vapC Uniprot ID A0A1U9Z9S7
Locus tag Mame_RS25715 Protein ID WP_018067750.1
Coordinates 5169..5591 (-) Length 141 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1U9Z9P8
Locus tag Mame_RS25720 Protein ID WP_018067751.1
Coordinates 5588..5854 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Mame_RS25695 1279..2265 + 987 WP_018067138.1 plasmid partitioning protein RepB -
Mame_RS25700 2424..3743 + 1320 WP_018067137.1 replication initiation protein RepC -
Mame_RS25705 3820..4512 - 693 WP_026173981.1 YafY family transcriptional regulator -
Mame_RS25710 4629..5048 + 420 WP_018067749.1 DUF1761 family protein -
Mame_RS25715 5169..5591 - 423 WP_018067750.1 type II toxin-antitoxin system VapC family toxin Toxin
Mame_RS25720 5588..5854 - 267 WP_018067751.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
Mame_RS25725 5999..7069 - 1071 WP_018067752.1 site-specific integrase -
Mame_RS25730 7216..8169 + 954 WP_026173983.1 DUF1403 family protein -
Mame_RS25735 8172..8873 + 702 WP_018067754.1 SMC-Scp complex subunit ScpB -
Mame_RS25740 8940..9770 - 831 WP_018067755.1 DUF3883 domain-containing protein -
Mame_RS25745 9816..10139 - 324 WP_018067756.1 DNA-binding transcriptional regulator -
Mame_RS25750 10129..10488 - 360 WP_026173985.1 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..169914 169914


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(8-73)


Sequences

Toxin        


Download         Length: 141 a.a.        Molecular weight: 15537.78 Da        Isoelectric Point: 4.6616

>T74225 WP_018067750.1 NZ_CP020333:c5591-5169 [Martelella mediterranea DSM 17316]
VKLLLDTNVLSEVTKPRPENRVLAWLDEIDEDRSFISVVSLAEIRRGVALMDSGRRRDALADWLANDLPQRFEQRVLAVD
EDVAFAWGDLMGDAKRRGRGMSSMDGLIAATAMAHGLTLATRNIKDFTGCGIELIDPWAV

Download         Length: 423 bp

>T74225 NZ_CP020333:c5591-5169 [Martelella mediterranea DSM 17316]
GTGAAGCTCCTGCTCGACACCAACGTCCTGTCTGAAGTGACGAAGCCGCGCCCGGAGAACCGCGTTCTTGCATGGCTGGA
TGAGATTGACGAGGATCGCTCCTTTATCAGTGTCGTGTCGCTTGCCGAAATCCGTCGCGGTGTCGCGCTCATGGACAGCG
GGCGAAGGCGCGATGCTCTTGCCGATTGGCTTGCCAACGACCTGCCGCAACGTTTCGAGCAACGCGTGCTTGCCGTGGAC
GAAGACGTGGCTTTCGCCTGGGGCGACCTGATGGGGGACGCAAAGCGCCGTGGACGCGGAATGTCGTCGATGGACGGGCT
GATCGCGGCCACGGCAATGGCACACGGGCTCACCCTTGCGACACGCAATATCAAGGATTTCACGGGTTGTGGCATAGAGC
TGATCGATCCATGGGCCGTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9780.10 Da        Isoelectric Point: 8.7353

>AT74225 WP_018067751.1 NZ_CP020333:c5854-5588 [Martelella mediterranea DSM 17316]
MQPVSHDEKNWTVAKAKAKLSEVIERAQSTPQTITRNGKPSVVVVSAEEWQRKVARKGTLAEFLLASPLRGAELDLDRQG
DEPRDLSL

Download         Length: 267 bp

>AT74225 NZ_CP020333:c5854-5588 [Martelella mediterranea DSM 17316]
ATGCAGCCCGTATCGCACGATGAAAAGAACTGGACGGTCGCCAAGGCAAAGGCCAAGCTTTCAGAGGTGATTGAGCGTGC
GCAATCCACGCCACAGACAATTACACGCAACGGCAAGCCGAGCGTCGTGGTGGTCTCCGCCGAAGAATGGCAGCGGAAGG
TCGCGCGCAAGGGGACCCTGGCCGAGTTTCTTCTGGCGTCCCCGCTGCGTGGCGCCGAGCTTGATCTCGACCGGCAGGGC
GACGAGCCACGCGATCTATCGCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1U9Z9S7

Antitoxin

Source ID Structure
AlphaFold DB A0A1U9Z9P8

References