Detailed information of TA system TA074223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 89411..90072
• Replicon: plasmid pMM259
• Accession: NZ_CP020332
• Organism: Martelella mediterranea DSM 17316 strain MACL11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Mame_RS24820
• Protein ID: WP_018067312.1
• Coordinates: 89411..89815 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1U9Z978
• Locus tag: Mame_RS24825
• Protein ID: WP_018067313.1
• Coordinates: 89812..90072 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mame_RS24815	89200..89430	-	231	WP_155122296.1	hypothetical protein	-
Mame_RS24820	89411..89815	-	405	WP_018067312.1	type II toxin-antitoxin system VapC family toxin	Toxin
Mame_RS24825	89812..90072	-	261	WP_018067313.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Mame_RS24830	90133..90690	-	558	WP_026173914.1	hypothetical protein	-
Mame_RS24835	90817..92925	-	2109	WP_157624605.1	ParB/RepB/Spo0J family partition protein	-
Mame_RS24840	93069..93965	-	897	WP_018067316.1	DUF1738 domain-containing protein	-
Mame_RS24845	94134..94765	+	632	Protein_81	SOS response-associated peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..258597	258597

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (15-51)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14091.34 Da
• Isoelectric Point: 5.5857

>T74223 WP_018067312.1 NZ_CP020332:c89815-89411 [Martelella mediterranea DSM 17316]
MSRLYMLDTNIVSNLARNPAGAVVGHIADVGQDTICVSIITAAELCYGCERKGSAKLTAQVEAILGGMQILALDAPAHAR
YGRIRAGLEAAGTPIGPNDLLIAAHALAVDAVLVTANMREFLRVPGLQVENWLD

Nucleotide

• Length: 405 bp

>T74223 NZ_CP020332:c89815-89411 [Martelella mediterranea DSM 17316]
TTGAGCCGCCTTTACATGCTCGACACGAATATCGTGTCGAATCTCGCGCGTAATCCGGCCGGCGCCGTCGTCGGCCATAT
CGCTGATGTCGGGCAGGATACGATCTGTGTCAGCATCATCACCGCGGCTGAGCTATGCTATGGCTGCGAACGCAAGGGAT
CTGCCAAGCTGACGGCCCAGGTCGAGGCCATTCTTGGCGGCATGCAAATACTCGCATTGGATGCGCCAGCACATGCCCGG
TACGGCCGCATCAGAGCAGGTCTCGAAGCAGCCGGTACGCCGATCGGTCCAAATGATCTGTTGATTGCAGCCCATGCCTT
GGCTGTCGATGCTGTTCTGGTGACAGCCAATATGCGCGAATTTCTGCGCGTGCCCGGTCTCCAGGTTGAAAACTGGCTGG
ACTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9792.24 Da
• Isoelectric Point: 4.8232

>AT74223 WP_018067313.1 NZ_CP020332:c90072-89812 [Martelella mediterranea DSM 17316]
MPQPQVFEAREAKLFRNNKSQAVRIPADFELPGDRVLIHREGNRLVIEPAPNKNLLDILARLKPLGPDDQFPDVDETLLP
AREIDL

Nucleotide

• Length: 261 bp

>AT74223 NZ_CP020332:c90072-89812 [Martelella mediterranea DSM 17316]
ATGCCCCAACCCCAAGTATTTGAAGCACGCGAAGCCAAGCTGTTTCGCAACAATAAAAGTCAGGCGGTTCGTATCCCGGC
CGATTTCGAGTTGCCTGGCGACCGTGTGCTGATACACCGGGAGGGAAACCGGCTTGTGATCGAACCTGCCCCCAACAAAA
ATCTTCTGGACATTCTGGCGCGACTGAAACCGCTCGGCCCTGATGACCAGTTTCCCGATGTCGATGAGACCCTGCTGCCA
GCAAGGGAAATCGACCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1U9Z978