Detailed information of TA system TA074221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1760225..1760880
• Replicon: chromosome
• Accession: NZ_CP020330
• Organism: Martelella mediterranea DSM 17316 strain MACL11

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Mame_RS08190
• Protein ID: WP_018066224.1
• Coordinates: 1760225..1760629 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1U9YZV0
• Locus tag: Mame_RS08195
• Protein ID: WP_018066223.1
• Coordinates: 1760626..1760880 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Mame_RS08160	1755329..1756277	+	949	WP_155122056.1	IS630 family transposase	-
Mame_RS08170	1756970..1757491	-	522	WP_018067869.1	hypothetical protein	-
Mame_RS08175	1757488..1758048	-	561	WP_018067868.1	phosphatase PAP2 family protein	-
Mame_RS08180	1758317..1759504	+	1188	WP_018067867.1	helix-turn-helix transcriptional regulator	-
Mame_RS26635	1759501..1759824	-	324	WP_155122202.1	transposase	-
Mame_RS26640	1759901..1760209	-	309	WP_081612572.1	transposase	-
Mame_RS08190	1760225..1760629	-	405	WP_018066224.1	type II toxin-antitoxin system VapC family toxin	Toxin
Mame_RS08195	1760626..1760880	-	255	WP_018066223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Mame_RS08200	1761307..1761921	-	615	WP_018066222.1	class I SAM-dependent methyltransferase	-
Mame_RS08205	1761934..1762851	-	918	WP_018066221.1	NAD(P)/FAD-dependent oxidoreductase	-
Mame_RS08210	1762986..1763474	+	489	WP_018066220.1	Rrf2 family transcriptional regulator	-
Mame_RS08215	1763529..1765421	-	1893	WP_018066219.1	PhoX family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (13-50)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14244.41 Da
• Isoelectric Point: 5.0839

>T74221 WP_018066224.1 NZ_CP020330:c1760629-1760225 [Martelella mediterranea DSM 17316]
MSRLYMLDTNIVSDLARNPAGAVVGHIVDVGQDTICVSIITAAELSYGCEHKGSARLTTQIEAILGGMQILALDAPAHAR
YGRIRAELEAAGTPIGPNELLIAAHALAVDAMLVTANTREFLRVPGLQVENWLN

Nucleotide

• Length: 405 bp

>T74221 NZ_CP020330:c1760629-1760225 [Martelella mediterranea DSM 17316]
TTGAGCCGTCTTTACATGCTCGACACGAATATCGTGTCGGATCTCGCGCGTAATCCGGCCGGCGCCGTCGTCGGCCATAT
CGTTGATGTCGGACAGGATACGATCTGCGTCAGCATCATCACCGCGGCTGAACTAAGCTATGGTTGTGAACACAAGGGAT
CTGCCAGGCTGACGACCCAGATCGAGGCCATTCTTGGCGGCATGCAAATACTCGCATTGGATGCCCCGGCACATGCCCGT
TACGGCCGCATCAGAGCAGAGCTCGAAGCTGCCGGCACGCCGATCGGTCCAAATGAACTGCTGATTGCGGCCCATGCCTT
GGCTGTCGATGCCATGCTGGTGACAGCCAATACGCGCGAATTCCTGCGGGTGCCCGGCCTCCAGGTTGAAAACTGGCTAA
ACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9510.95 Da
• Isoelectric Point: 5.1409

>AT74221 WP_018066223.1 NZ_CP020330:c1760880-1760626 [Martelella mediterranea DSM 17316]
MPQASEAREAKLFRNNKSQAVRIPADFELPGDRVMIHREGNRLVIEPAPKNKLLDILARLKPLGPDDQFPDVDETLLPAR
EIDL

Nucleotide

• Length: 255 bp

>AT74221 NZ_CP020330:c1760880-1760626 [Martelella mediterranea DSM 17316]
ATGCCCCAGGCATCTGAAGCACGTGAAGCCAAACTGTTTCGCAACAACAAAAGTCAGGCGGTCCGTATCCCGGCCGATTT
CGAACTGCCGGGCGACCGTGTGATGATTCATCGTGAGGGAAACCGACTGGTAATCGAGCCCGCCCCCAAAAACAAGCTTC
TGGACATTCTGGCGCGGCTGAAACCGCTCGGCCCGGATGACCAGTTCCCCGATGTCGATGAGACCTTGCTGCCAGCAAGG
GAAATCGACCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A1U9YZV0