Detailed information of TA system TA074219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 17597..18162
• Replicon: plasmid pPLP1
• Accession: NZ_CP020328
• Organism: Paenibacillus larvae subsp. pulvifaciens strain CCM 38

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B5S25_RS22550
• Protein ID: WP_077997739.1
• Coordinates: 17833..18162 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B5S25_RS22545
• Protein ID: WP_077997741.1
• Coordinates: 17597..17836 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S25_RS22520	13381..13827	+	447	WP_077997735.1	hypothetical protein	-
B5S25_RS22525	13941..14201	-	261	Protein_17	transposase	-
B5S25_RS22530	14382..15839	+	1458	WP_077997736.1	SIR2 family protein	-
B5S25_RS22535	15877..16551	+	675	WP_077997737.1	TIR domain-containing protein	-
B5S25_RS22540	16712..16963	-	252	WP_077997738.1	hypothetical protein	-
B5S25_RS23860	16971..17117	-	147	WP_155121122.1	hypothetical protein	-
B5S25_RS22545	17597..17836	+	240	WP_077997741.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B5S25_RS22550	17833..18162	+	330	WP_077997739.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B5S25_RS22555	18407..19045	-	639	WP_077997682.1	hemolysin III family protein	-
B5S25_RS22560	19813..20091	-	279	WP_077997683.1	HU family DNA-binding protein	-
B5S25_RS22565	20201..20407	-	207	WP_077997684.1	cold-shock protein	-
B5S25_RS22570	20764..21447	+	684	WP_077996502.1	helix-turn-helix domain-containing protein	-
B5S25_RS22575	21585..22013	+	429	WP_077997685.1	IS3 family transposase	-
B5S25_RS22580	22010..22336	+	327	WP_077997686.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60855	60855

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-108)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12407.45 Da
• Isoelectric Point: 7.3587

>T74219 WP_077997739.1 NZ_CP020328:17833-18162 [Paenibacillus larvae subsp. pulvifaciens]
VTVVPDRGDIVYMDFNPQSGHEQAGWRPALVLSPKLFNATTNLAVVCPITSRIKGYLFEVHLPDAMMTKGVILVHQLKSA
DWRSRRLEIREKAPQEIIDEVIEKIHTFL

Nucleotide

• Length: 330 bp

>T74219 NZ_CP020328:17833-18162 [Paenibacillus larvae subsp. pulvifaciens]
GTGACAGTTGTTCCAGATCGCGGTGATATCGTTTATATGGACTTTAATCCACAGAGCGGACATGAACAAGCCGGGTGGCG
ACCAGCCCTTGTACTGTCACCAAAATTATTTAATGCTACAACGAATCTTGCTGTAGTCTGCCCTATCACTTCTAGAATTA
AAGGCTACTTATTTGAAGTTCATCTTCCAGACGCAATGATGACAAAAGGTGTTATACTTGTGCACCAATTGAAAAGCGCC
GATTGGAGATCGCGGAGATTAGAAATTAGGGAGAAAGCACCACAAGAAATTATTGACGAAGTGATTGAAAAGATTCATAC
TTTCCTATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8957.25 Da
• Isoelectric Point: 5.6987

>AT74219 WP_077997741.1 NZ_CP020328:17597-17836 [Paenibacillus larvae subsp. pulvifaciens]
MSTLVSKWGNSLALRIPRHFAESLNLNEGSEVELKLTEDCLSVKPKKRKYTLEELCASIKDGNQHDLIDFGKPVGEEIW

Nucleotide

• Length: 240 bp

>AT74219 NZ_CP020328:17597-17836 [Paenibacillus larvae subsp. pulvifaciens]
ATGAGCACGCTTGTAAGCAAATGGGGAAATAGTCTGGCACTTCGTATTCCCAGACATTTTGCTGAAAGCTTAAATCTTAA
TGAAGGCTCTGAAGTTGAATTAAAGCTAACAGAAGACTGCTTGAGTGTCAAGCCGAAGAAGAGAAAGTATACCCTTGAGG
AACTTTGTGCTTCTATTAAAGACGGTAATCAGCATGACCTTATTGATTTCGGTAAACCTGTTGGGGAGGAAATTTGGTGA