Detailed information of TA system TA074218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1309856..1310451
• Replicon: chromosome
• Accession: NZ_CP020327
• Organism: Paenibacillus larvae subsp. pulvifaciens strain CCM 38

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B5S25_RS06995
• Protein ID: WP_077997555.1
• Coordinates: 1310158..1310451 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W2E434
• Locus tag: B5S25_RS06990
• Protein ID: WP_036658190.1
• Coordinates: 1309856..1310125 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S25_RS06970	1304869..1305483	+	615	WP_077996009.1	sigma-70 family RNA polymerase sigma factor	-
B5S25_RS06975	1305515..1307431	-	1917	WP_077996010.1	AAA family ATPase	-
B5S25_RS06980	1307691..1308977	+	1287	WP_077996011.1	MFS transporter	-
B5S25_RS06985	1309205..1309537	-	333	WP_077996012.1	hypothetical protein	-
B5S25_RS06990	1309856..1310125	+	270	WP_036658190.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B5S25_RS06995	1310158..1310451	+	294	WP_077997555.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B5S25_RS07000	1310703..1310993	+	291	WP_023483432.1	metalloregulator ArsR/SmtB family transcription factor	-
B5S25_RS07005	1310990..1313500	+	2511	WP_077996013.1	FAD-dependent oxidoreductase	-
B5S25_RS07010	1313605..1313901	+	297	WP_036658187.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1315004..1316227	1223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-96)

Antitoxin

• Antitoxin PemI-like (11-89)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10996.75 Da
• Isoelectric Point: 7.2775

>T74218 WP_077997555.1 NZ_CP020327:1310158-1310451 [Paenibacillus larvae subsp. pulvifaciens]
MDFNPQIGHEQAGRRPGLVVSPTSFNRVTGFIVVCPITRTVRNYPYEVPLPSDLMTEGVVLAHQVKSLDWKARKAEVREK
VPKEIIDDVLDLIHTFL

Nucleotide

• Length: 294 bp

>T74218 NZ_CP020327:1310158-1310451 [Paenibacillus larvae subsp. pulvifaciens]
ATGGATTTTAACCCGCAGATTGGACATGAACAAGCCGGAAGAAGACCAGGCCTTGTTGTCTCTCCTACATCATTCAATAG
GGTAACAGGCTTTATAGTTGTATGCCCTATTACAAGAACCGTTAGGAATTATCCATATGAAGTTCCCCTCCCTTCCGATC
TTATGACCGAAGGTGTTGTTTTGGCTCATCAAGTAAAAAGCTTGGATTGGAAGGCAAGAAAGGCAGAAGTTCGGGAAAAA
GTACCAAAAGAAATTATTGACGATGTACTGGATTTAATTCACACTTTCCTATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10006.46 Da
• Isoelectric Point: 5.7379

>AT74218 WP_036658190.1 NZ_CP020327:1309856-1310125 [Paenibacillus larvae subsp. pulvifaciens]
MGKDRERMSVMSTIVSKWGNSLAIRIPSNLAESLNIKEGSEVELSIMDSTLTIKPKKRKPTLEELLAKITDGNHHEEIDF
GKPEGNEVW

Nucleotide

• Length: 270 bp

>AT74218 NZ_CP020327:1309856-1310125 [Paenibacillus larvae subsp. pulvifaciens]
ATGGGTAAAGACAGGGAAAGGATGAGTGTAATGAGCACAATTGTGAGCAAATGGGGAAACAGTCTCGCAATCCGCATTCC
AAGTAACCTGGCAGAAAGCCTAAACATAAAAGAGGGTTCTGAAGTTGAATTAAGCATCATGGATAGTACACTAACTATAA
AACCTAAAAAACGTAAGCCAACTCTCGAAGAATTACTGGCAAAAATAACAGACGGTAACCATCATGAGGAAATTGACTTT
GGAAAACCGGAGGGGAACGAGGTTTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1TQJ8