Detailed information of TA system TA074217

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 1027535..1028179
• Replicon: chromosome
• Accession: NZ_CP020327
• Organism: Paenibacillus larvae subsp. pulvifaciens strain CCM 38

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W2E6W9
• Locus tag: B5S25_RS05440
• Protein ID: WP_036654551.1
• Coordinates: 1027535..1027738 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: B5S25_RS05445
• Protein ID: WP_077995836.1
• Coordinates: 1027769..1028179 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5S25_RS05425	1023791..1024012	-	222	WP_077995833.1	hypothetical protein	-
B5S25_RS05430	1024029..1025891	-	1863	WP_077995834.1	phage terminase large subunit family protein	-
B5S25_RS05435	1025875..1026387	-	513	WP_077995835.1	hypothetical protein	-
B5S25_RS05440	1027535..1027738	+	204	WP_036654551.1	type II toxin-antitoxin system HicA family toxin	Toxin
B5S25_RS05445	1027769..1028179	+	411	WP_077995836.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
B5S25_RS05450	1028208..1028411	-	204	WP_077995837.1	hypothetical protein	-
B5S25_RS23275	1028592..1028741	-	150	Protein_1097	response regulator transcription factor	-
B5S25_RS05460	1028882..1030192	-	1311	WP_024094814.1	purine/pyrimidine permease	-
B5S25_RS05465	1030331..1031263	-	933	WP_036654839.1	dihydroorotate dehydrogenase	-
B5S25_RS05470	1031244..1031945	-	702	WP_077995841.1	dihydroorotate dehydrogenase electron transfer subunit	-
B5S25_RS23980	1032173..1032277	-	105	WP_188318623.1	sporulation protein YjcZ	-
B5S25_RS05475	1032361..1032888	+	528	WP_077997537.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	926221..1056119	129898

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (10-66)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-107)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 7774.08 Da
• Isoelectric Point: 10.8900

>T74217 WP_036654551.1 NZ_CP020327:1027535-1027738 [Paenibacillus larvae subsp. pulvifaciens]
MVNLNKAYSSREVIKILKEDGWIHKNTEGDHWHFIHPTKKGKVTVPHPKKGLKRRTTKSIFEQAGLI

Nucleotide

• Length: 204 bp

>T74217 NZ_CP020327:1027535-1027738 [Paenibacillus larvae subsp. pulvifaciens]
ATGGTCAACCTCAATAAAGCATACTCATCAAGAGAGGTTATCAAAATCCTAAAAGAGGATGGCTGGATACATAAAAACAC
CGAAGGCGATCATTGGCACTTTATTCATCCCACTAAAAAAGGAAAGGTGACTGTACCCCATCCGAAAAAAGGCTTGAAAC
GTCGAACAACAAAAAGTATCTTTGAGCAGGCAGGGCTGATTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15121.91 Da
• Isoelectric Point: 4.0257

>AT74217 WP_077995836.1 NZ_CP020327:1027769-1028179 [Paenibacillus larvae subsp. pulvifaciens]
MKYIYPAVFTPGEPDEGGFTVTFPDLPGCITEGDDLEEALHMAKDALGGHLYLMEEDGDEIPRSSQPDSIQLETGEFVSL
IQANTDFIRERIQSKAVNKTVTLPKWLNDVAAEEGINFSQTLQDALKQKLEIKDFS

Nucleotide

• Length: 411 bp

>AT74217 NZ_CP020327:1027769-1028179 [Paenibacillus larvae subsp. pulvifaciens]
ATGAAATACATTTACCCTGCTGTTTTTACCCCCGGCGAACCAGACGAAGGAGGCTTTACTGTCACATTCCCTGATCTTCC
TGGATGCATCACAGAAGGCGATGATCTGGAAGAAGCCCTACACATGGCAAAAGATGCTCTTGGCGGCCATTTATACCTGA
TGGAAGAGGATGGCGACGAAATACCTCGTTCATCACAACCAGACTCTATACAGCTTGAAACTGGAGAATTTGTTTCTCTT
ATTCAGGCTAATACAGATTTCATCCGCGAAAGGATTCAGAGTAAAGCCGTGAATAAGACCGTCACTCTTCCAAAATGGCT
TAATGATGTAGCAGCGGAAGAAGGCATTAACTTTTCTCAGACTCTTCAGGATGCATTAAAACAAAAGCTTGAAATCAAAG
ACTTCTCCTAA

Structures

Toxin

• AlphaFold DB: A0A2L1UH89