Detailed information of TA system TA074216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 1772093..1772595
• Replicon: chromosome
• Accession: NZ_CP020121
• Organism: Comamonas kerstersii strain 8943

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q5XQD8
• Locus tag: B5M06_RS08295
• Protein ID: WP_032492620.1
• Coordinates: 1772341..1772595 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0BEH6
• Locus tag: B5M06_RS08290
• Protein ID: WP_032492621.1
• Coordinates: 1772093..1772344 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5M06_RS08265	1767948..1768919	-	972	WP_054065446.1	dihydropteroate synthase	-
B5M06_RS08270	1768913..1769260	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
B5M06_RS08275	1769424..1770215	-	792	WP_001206316.1	ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1	-
B5M06_RS08280	1770328..1771158	-	831	WP_001334766.1	oxacillin-hydrolyzing class D beta-lactamase OXA-1	-
B5M06_RS08285	1771277..1771909	-	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-
B5M06_RS08290	1772093..1772344	+	252	WP_032492621.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
B5M06_RS08295	1772341..1772595	+	255	WP_032492620.1	Txe/YoeB family addiction module toxin	Toxin
B5M06_RS08300	1772668..1773222	-	555	WP_003159191.1	aminoglycoside N-acetyltransferase AAC(6')-Ib4	-
B5M06_RS08305	1773368..1773841	-	474	WP_001389366.1	trimethoprim-resistant dihydrofolate reductase DfrA17	-
B5M06_RS08310	1773923..1774477	-	555	WP_003159191.1	aminoglycoside N-acetyltransferase AAC(6')-Ib4	-
B5M06_RS08315	1774830..1775291	+	462	WP_074424383.1	phage integrase N-terminal SAM-like domain-containing protein	-
B5M06_RS08320	1775518..1776864	+	1347	WP_080025203.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(A) / aph(6)-Id / aph(3'')-Ib / floR / qacE / ant(3'')-Ia / blaOXA-1 / catB3 / sul2	-	1758744..1792687	33943
	-	flank	IS/Tn	tet(A) / aph(6)-Id / aph(3'')-Ib / floR / qacE / ant(3'')-Ia / blaOXA-1 / catB3	-	1758744..1771909	13165
	-	inside	Integron	-	-	1768913..1774606	5693

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10272.81 Da
• Isoelectric Point: 9.3585

>T74216 WP_032492620.1 NZ_CP020121:1772341-1772595 [Comamonas kerstersii]
MRLIFSDDAWEDYLFWQKQDRRMVDRINKLIKETTREPFSGVGKPEPLKHALAGYWSRRITDEHRMVYKVADDALWIVQL
KYHY

Nucleotide

• Length: 255 bp

>T74216 NZ_CP020121:1772341-1772595 [Comamonas kerstersii]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9244.40 Da
• Isoelectric Point: 4.7769

>AT74216 WP_032492621.1 NZ_CP020121:1772093-1772344 [Comamonas kerstersii]
MDAITYTHARANLAGTMDRVCNDHEPVIITRNGDQSVVILSLEDFQALEETAYLLRSPANAKRLFKSIEQLEAGRGQARE
LAE

Nucleotide

• Length: 252 bp

>AT74216 NZ_CP020121:1772093-1772344 [Comamonas kerstersii]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Structures

Toxin

• AlphaFold DB: A0A1V0BEH9

Antitoxin

• AlphaFold DB: A0A1V0BEH6