Detailed information of TA system TA074213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 86344..86945
• Replicon: plasmid unitig_5
• Accession: NZ_CP020117
• Organism: Escherichia coli strain AR_0104

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: AM483_RS26410
• Protein ID: WP_001216034.1
• Coordinates: 86344..86724 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: AM483_RS26415
• Protein ID: WP_001190712.1
• Coordinates: 86724..86945 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM483_RS26360	81929..82744	-	816	WP_012372823.1	Na(+)-translocating NADH-quinone reductase subunit C	-
AM483_RS26365	82927..83433	-	507	WP_000949452.1	protein disulfide oxidoreductase	-
AM483_RS26370	83423..83581	-	159	WP_000449408.1	DsbA family protein	-
AM483_RS28550	83731..83844	-	114	Protein_97	IS6 family transposase	-
AM483_RS26385	84044..84741	+	698	Protein_98	IS1 family transposase	-
AM483_RS28090	84756..84974	+	219	Protein_99	IS4 family transposase	-
AM483_RS26395	85024..85728	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
AM483_RS26400	85740..86132	-	393	WP_080029215.1	pdcB	-
AM483_RS26405	86160..86339	-	180	WP_001513661.1	hypothetical protein	-
AM483_RS26410	86344..86724	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
AM483_RS26415	86724..86945	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
AM483_RS26420	87128..88684	+	1557	WP_001617892.1	type I restriction-modification system subunit M	-
AM483_RS26425	88681..89952	+	1272	WP_023142242.1	restriction endonuclease subunit S	-
AM483_RS26440	90203..90900	+	698	Protein_107	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5	senB	1..107210	107210
	-	inside	IScluster/Tn	-	-	84238..90900	6662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T74213 WP_001216034.1 NZ_CP020117:c86724-86344 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T74213 NZ_CP020117:c86724-86344 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT74213 WP_001190712.1 NZ_CP020117:c86945-86724 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT74213 NZ_CP020117:c86945-86724 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6