Detailed information of TA system TA074204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4979684..4980484
• Replicon: chromosome
• Accession: NZ_CP020116
• Organism: Escherichia coli strain AR_0104

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: AM483_RS25715
• Protein ID: WP_000342452.1
• Coordinates: 4979957..4980484 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: AM483_RS25710
• Protein ID: WP_001277107.1
• Coordinates: 4979684..4979950 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM483_RS25690	4975343..4976011	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
AM483_RS25695	4976004..4977062	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
AM483_RS25700	4977307..4978161	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
AM483_RS25705	4978432..4979535	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
AM483_RS25710	4979684..4979950	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
AM483_RS25715	4979957..4980484	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
AM483_RS25720	4980481..4980864	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
AM483_RS25725	4981287..4982396	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
AM483_RS25730	4982444..4983370	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
AM483_RS25735	4983367..4984644	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
AM483_RS25740	4984641..4985408	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T74204 WP_000342452.1 NZ_CP020116:4979957-4980484 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T74204 NZ_CP020116:4979957-4980484 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT74204 WP_001277107.1 NZ_CP020116:4979684-4979950 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT74204 NZ_CP020116:4979684-4979950 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57