Detailed information of TA system TA074199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 3931012..3931832
• Replicon: chromosome
• Accession: NZ_CP020116
• Organism: Escherichia coli strain AR_0104

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: AM483_RS20455
• Protein ID: WP_001054379.1
• Coordinates: 3931012..3931269 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: AM483_RS20460
• Protein ID: WP_000123957.1
• Coordinates: 3931281..3931832 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM483_RS20430	3926559..3927539	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
AM483_RS20440	3928122..3929228	-	1107	Protein_3776	helicase YjhR	-
AM483_RS20445	3929291..3930438	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
AM483_RS20450	3930471..3930635	+	165	Protein_3778	GNAT family N-acetyltransferase	-
AM483_RS20455	3931012..3931269	+	258	WP_001054379.1	YjhX family toxin	Toxin
AM483_RS20460	3931281..3931832	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
AM483_RS20465	3931884..3932630	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
AM483_RS20470	3932758..3933018	+	261	WP_000077645.1	hypothetical protein	-
AM483_RS28510	3933056..3933172	+	117	Protein_3783	VOC family protein	-
AM483_RS20480	3933417..3934538	+	1122	WP_000010833.1	M42 family metallopeptidase	-
AM483_RS20485	3934535..3934813	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
AM483_RS20490	3934825..3936138	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T74199 WP_001054379.1 NZ_CP020116:3931012-3931269 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T74199 NZ_CP020116:3931012-3931269 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT74199 WP_000123957.1 NZ_CP020116:3931281-3931832 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT74199 NZ_CP020116:3931281-3931832 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030