Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3319125..3319743 Replicon chromosome
Accession NZ_CP020116
Organism Escherichia coli strain AR_0104

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag AM483_RS17385 Protein ID WP_001291435.1
Coordinates 3319525..3319743 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag AM483_RS17380 Protein ID WP_000344800.1
Coordinates 3319125..3319499 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM483_RS17370 3314214..3315407 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
AM483_RS17375 3315430..3318579 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
AM483_RS17380 3319125..3319499 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
AM483_RS17385 3319525..3319743 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
AM483_RS17390 3319917..3320468 + 552 WP_000102539.1 maltose O-acetyltransferase -
AM483_RS17395 3320584..3321054 + 471 WP_000136192.1 YlaC family protein -
AM483_RS17400 3321218..3322768 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
AM483_RS17405 3322810..3323163 - 354 WP_000878135.1 DUF1428 family protein -
AM483_RS17415 3323542..3323853 + 312 WP_000409908.1 MGMT family protein -
AM483_RS17420 3323884..3324456 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74195 WP_001291435.1 NZ_CP020116:3319525-3319743 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74195 NZ_CP020116:3319525-3319743 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT74195 WP_000344800.1 NZ_CP020116:3319125-3319499 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74195 NZ_CP020116:3319125-3319499 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References