Detailed information of TA system TA074178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 5271363..5272060
• Replicon: chromosome
• Accession: NZ_CP020114
• Organism: Nodularia spumigena UHCC 0039

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A2S0QBG5
• Locus tag: BMF81_RS23630
• Protein ID: WP_006196010.1
• Coordinates: 5271686..5272060 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: BMF81_RS23625
• Protein ID: WP_006196011.1
• Coordinates: 5271363..5271689 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF81_RS23595	5266485..5267753	-	1269	WP_006196016.1	tetratricopeptide repeat protein	-
BMF81_RS23600	5267899..5269047	+	1149	WP_006196015.1	DUF711 family protein	-
BMF81_RS23610	5269180..5269851	-	672	WP_006196014.1	2OG-Fe dioxygenase family protein	-
BMF81_RS23615	5270072..5270797	+	726	WP_006196013.1	DNA/RNA nuclease SfsA	-
BMF81_RS23620	5270856..5271341	+	486	WP_107806969.1	gluconokinase	-
BMF81_RS23625	5271363..5271689	-	327	WP_006196011.1	helix-turn-helix domain-containing protein	Antitoxin
BMF81_RS23630	5271686..5272060	-	375	WP_006196010.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BMF81_RS23635	5272208..5273185	-	978	WP_107806970.1	carbohydrate kinase	-
BMF81_RS23640	5273537..5273800	+	264	WP_006196008.1	helix-turn-helix transcriptional regulator	-
BMF81_RS23645	5273985..5274614	+	630	WP_006196007.1	hypothetical protein	-
BMF81_RS23650	5274709..5275704	+	996	WP_006196006.1	glycosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (24-115)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-89)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14061.19 Da
• Isoelectric Point: 9.4945

>T74178 WP_006196010.1 NZ_CP020114:c5272060-5271686 [Nodularia spumigena UHCC 0039]
MADDEDIIEIPLRPLVWMGDSLKNIRSFPEQVRASVGYALQLVQAGETPMDAKPFKGVGSGVYEIVKRYDTDTYRAVYAV
KIGEKIYVLHAFQKKSKQGIKTPQADVDLIKQRYKDAVAREKQQ

Nucleotide

• Length: 375 bp

>T74178 NZ_CP020114:c5272060-5271686 [Nodularia spumigena UHCC 0039]
ATGGCAGATGATGAGGATATTATCGAAATTCCTCTACGACCTCTGGTTTGGATGGGAGACTCTCTCAAAAATATTCGCTC
ATTTCCTGAACAAGTCCGTGCATCAGTAGGCTACGCACTGCAATTGGTGCAAGCAGGGGAAACACCGATGGATGCCAAGC
CTTTTAAGGGGGTTGGAAGTGGCGTGTATGAAATAGTCAAACGCTACGATACTGATACTTACAGGGCAGTTTATGCGGTA
AAGATTGGTGAAAAAATCTATGTCCTGCACGCTTTTCAAAAGAAATCAAAGCAAGGAATTAAAACCCCACAGGCTGATGT
TGATCTGATTAAACAACGCTATAAGGACGCAGTAGCTAGGGAAAAACAACAATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12286.19 Da
• Isoelectric Point: 7.3216

>AT74178 WP_006196011.1 NZ_CP020114:c5271689-5271363 [Nodularia spumigena UHCC 0039]
MTQESVFEESSGNVFADLGLSDADELFTRGKIGIQVLRLLKQRNLKQREISKLLGIPQPEVSHLMKGEFQRFSEGKLLIF
LKRLDTEITLHLRPRHAPDQSAETVISL

Nucleotide

• Length: 327 bp

>AT74178 NZ_CP020114:c5271689-5271363 [Nodularia spumigena UHCC 0039]
ATGACACAGGAAAGCGTTTTTGAAGAAAGTAGTGGTAATGTATTCGCTGACCTCGGTTTGTCAGATGCGGATGAACTTTT
TACACGGGGAAAGATAGGGATTCAGGTACTGCGGCTTTTGAAACAAAGGAACCTGAAACAACGTGAAATCAGCAAACTTC
TTGGTATTCCCCAGCCAGAAGTTTCTCATTTGATGAAAGGAGAGTTTCAACGGTTTAGCGAGGGTAAACTCCTCATTTTC
CTCAAGCGACTTGATACGGAAATCACTTTACATCTTCGCCCTCGTCACGCGCCAGATCAGTCTGCTGAAACGGTGATATC
CTTATAG

Structures

Toxin

• AlphaFold DB: A0A2S0QBG5