Detailed information of TA system TA074175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazE-MazF
• Location: 5171594..5172171
• Replicon: chromosome
• Accession: NZ_CP020114
• Organism: Nodularia spumigena UHCC 0039

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0ZGE9
• Locus tag: BMF81_RS23145
• Protein ID: WP_006196818.1
• Coordinates: 5171594..5171854 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BMF81_RS23150
• Protein ID: WP_006196817.1
• Coordinates: 5171842..5172171 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF81_RS23125	5166902..5169718	-	2817	WP_107806947.1	GUN4 domain-containing protein	-
BMF81_RS23130	5170134..5170964	+	831	WP_006196820.1	glycosyltransferase family 2 protein	-
BMF81_RS23140	5171265..5171381	-	117	WP_006196819.1	DUF4351 domain-containing protein	-
BMF81_RS23145	5171594..5171854	+	261	WP_006196818.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
BMF81_RS23150	5171842..5172171	+	330	WP_006196817.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
BMF81_RS23155	5172244..5175036	-	2793	WP_006196816.1	preprotein translocase subunit SecA	-
BMF81_RS23160	5175292..5175885	-	594	WP_006196815.1	cytochrome c	-
BMF81_RS23165	5175968..5176324	-	357	WP_006196814.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BMF81_RS23170	5176314..5176571	-	258	WP_006196813.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• SpoVT-AbrB domain (10-52)

Antitoxin

• mRNA interferase PemK-like (4-107)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9874.23 Da
• Isoelectric Point: 4.8380

>T74175 WP_006196818.1 NZ_CP020114:5171594-5171854 [Nodularia spumigena UHCC 0039]
MGKAIKTRIVKIGNSQGLRIPKILLEQSGINSEVEIEVHGNHLIIRPVEQARANWDKAFAAMAEKHDDILLDDINTTEWD
QVEWEW

Nucleotide

• Length: 261 bp

>T74175 NZ_CP020114:5171594-5171854 [Nodularia spumigena UHCC 0039]
ATGGGTAAAGCAATTAAAACCCGAATAGTAAAAATCGGCAATTCCCAAGGACTTCGTATACCCAAAATCCTATTAGAACA
AAGCGGTATTAACTCAGAAGTAGAAATTGAAGTTCATGGAAATCATCTGATTATTCGCCCAGTTGAACAAGCAAGGGCGA
ATTGGGACAAAGCATTTGCAGCAATGGCAGAAAAACACGATGATATCCTATTAGATGATATCAATACAACAGAATGGGAT
CAGGTTGAATGGGAGTGGTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12083.08 Da
• Isoelectric Point: 7.8631

>AT74175 WP_006196817.1 NZ_CP020114:5171842-5172171 [Nodularia spumigena UHCC 0039]
MGVVVNRFDVFLVSLDPTIGSEIQKTRPCLVISPNEINHHIATVIVAPMTTKGQTYPTRVTCQFQGQNGQIVLDQIRTVD
KTRLVKFIGQISVEAQKTVLDVLAQMFGE

Nucleotide

• Length: 330 bp

>AT74175 NZ_CP020114:5171842-5172171 [Nodularia spumigena UHCC 0039]
ATGGGAGTGGTAGTCAATCGCTTTGATGTTTTCCTAGTTAGTCTTGATCCTACTATCGGGAGTGAAATTCAAAAAACACG
CCCCTGTTTAGTAATTTCACCAAACGAGATCAACCACCATATTGCCACCGTAATTGTTGCACCAATGACTACAAAAGGAC
AAACATATCCAACAAGGGTAACTTGTCAATTTCAAGGACAAAATGGACAAATTGTACTTGACCAAATTCGCACAGTGGAT
AAAACTCGATTAGTCAAATTTATTGGTCAAATTAGTGTAGAAGCACAAAAAACAGTTCTTGATGTCCTAGCCCAAATGTT
TGGGGAATAA

Structures

Toxin

• AlphaFold DB: A0ZGE9