Detailed information of TA system TA074174

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 3481346..3481721
• Replicon: chromosome
• Accession: NZ_CP020114
• Organism: Nodularia spumigena UHCC 0039

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: BMF81_RS15525
• Protein ID: WP_081450159.1
• Coordinates: 3481551..3481721 (-)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A166J371
• Locus tag: BMF81_RS15520
• Protein ID: WP_006197472.1
• Coordinates: 3481346..3481570 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMF81_RS15500	3476730..3478097	-	1368	WP_107806575.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
BMF81_RS15505	3478660..3478755	-	96	WP_006197468.1	cytochrome b6-f complex subunit PetL	-
BMF81_RS15510	3478944..3480062	+	1119	WP_006197469.1	3-dehydroquinate synthase	-
BMF81_RS15515	3480844..3481221	+	378	WP_006197471.1	hypothetical protein	-
BMF81_RS15520	3481346..3481570	-	225	WP_006197472.1	CopG family transcriptional regulator	Antitoxin
BMF81_RS15525	3481551..3481721	-	171	WP_081450159.1	BrnT family toxin	Toxin
BMF81_RS15530	3481771..3482571	-	801	WP_006197473.1	IS4 family transposase	-
BMF81_RS15535	3483204..3484307	-	1104	WP_006197474.1	dihydroorotase	-
BMF81_RS15540	3484499..3484927	+	429	WP_006197475.1	RNA-directed DNA polymerase	-
BMF81_RS15545	3485006..3485869	+	864	WP_006197476.1	hypothetical protein	-
BMF81_RS15550	3486129..3486599	-	471	WP_006197477.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (2-47)

Antitoxin

• CopG antitoxin (7-69)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6704.74 Da
• Isoelectric Point: 10.1540

>T74174 WP_081450159.1 NZ_CP020114:c3481721-3481551 [Nodularia spumigena UHCC 0039]
IEIPARTEDEPRFLIVGVIAQKYWSAIITYRENRVRIVSIRRSRKEEVAIYESNRI

Nucleotide

• Length: 171 bp

>T74174 NZ_CP020114:c3481721-3481551 [Nodularia spumigena UHCC 0039]
ATTGAAATCCCCGCTCGAACCGAAGATGAACCCAGGTTTTTGATCGTCGGAGTGATTGCTCAAAAATACTGGTCAGCCAT
TATCACCTATCGAGAAAACAGAGTACGGATTGTCTCAATCCGCCGATCCCGAAAAGAAGAGGTAGCTATTTATGAAAGCA
ACAGAATTTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8693.94 Da
• Isoelectric Point: 4.8146

>AT74174 WP_006197472.1 NZ_CP020114:c3481570-3481346 [Nodularia spumigena UHCC 0039]
MKATEFDVKFDRGEDITEFLDLSQAQRPGLEQKRVNVDFPTWMIEALDREAKRLGVTRQSIIKIWLAERLEQVS

Nucleotide

• Length: 225 bp

>AT74174 NZ_CP020114:c3481570-3481346 [Nodularia spumigena UHCC 0039]
ATGAAAGCAACAGAATTTGATGTCAAATTTGATCGGGGAGAAGACATCACCGAATTTTTAGACCTTTCCCAAGCCCAACG
GCCTGGACTTGAGCAGAAACGAGTTAACGTTGATTTCCCCACCTGGATGATTGAAGCATTAGATCGTGAAGCAAAACGCT
TAGGGGTCACCCGTCAGTCAATTATTAAGATATGGCTTGCAGAACGGCTTGAGCAGGTAAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A166J371