Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 165100..165836 Replicon plasmid tig00000001
Accession NZ_CP020109
Organism Klebsiella pneumoniae strain AR_0098

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag AM477_RS28335 Protein ID WP_003026803.1
Coordinates 165100..165582 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag AM477_RS28340 Protein ID WP_003026799.1
Coordinates 165570..165836 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM477_RS28315 160737..161699 + 963 WP_004152113.1 M48 family metalloprotease -
AM477_RS29610 161787..163041 + 1255 Protein_173 IS3 family transposase -
AM477_RS28330 163542..164888 - 1347 WP_020314316.1 ISNCY family transposase -
AM477_RS28335 165100..165582 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
AM477_RS28340 165570..165836 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
AM477_RS28345 166012..166266 - 255 WP_004152108.1 hypothetical protein -
AM477_RS28350 166342..166599 - 258 WP_004152107.1 hypothetical protein -
AM477_RS28355 166648..166851 - 204 WP_004152106.1 hemolysin expression modulator Hha -
AM477_RS28360 166885..167253 - 369 WP_004152105.1 hypothetical protein -
AM477_RS28365 167297..167791 - 495 WP_004152104.1 DNA-binding protein -
AM477_RS28370 167822..168397 - 576 WP_004152103.1 hypothetical protein -
AM477_RS28375 168385..168654 - 270 WP_004152102.1 hypothetical protein -
AM477_RS28380 169012..169362 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
AM477_RS28385 169412..169774 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 / dfrA12 / aadA2 / qacE / sul1 / mph(A) - 1..169952 169952


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T74171 WP_003026803.1 NZ_CP020109:c165582-165100 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T74171 NZ_CP020109:c165582-165100 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT74171 WP_003026799.1 NZ_CP020109:c165836-165570 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT74171 NZ_CP020109:c165836-165570 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References