Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 3125426..3126012 Replicon chromosome
Accession NZ_CP020108
Organism Klebsiella pneumoniae strain AR_0098

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag AM477_RS15805 Protein ID WP_002920800.1
Coordinates 3125644..3126012 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag AM477_RS15800 Protein ID WP_002920802.1
Coordinates 3125426..3125647 (+) Length 74 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM477_RS15780 3121583..3122509 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
AM477_RS15785 3122506..3123783 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
AM477_RS15790 3123780..3124547 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
AM477_RS15795 3124549..3125262 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
AM477_RS15800 3125426..3125647 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
AM477_RS15805 3125644..3126012 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
AM477_RS15810 3126285..3127601 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
AM477_RS15815 3127708..3128595 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
AM477_RS15820 3128592..3129437 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
AM477_RS15825 3129439..3130509 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3086136..3131246 45110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(13-86)

Antitoxin

(1-49)


Sequences


Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T74159 WP_002920800.1 NZ_CP020108:3125644-3126012 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T74159 NZ_CP020108:3125644-3126012 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT74159 WP_002920802.1 NZ_CP020108:3125426-3125647 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT74159 NZ_CP020108:3125426-3125647 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References