Detailed information of TA system TA074149

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4079851..4080545
• Replicon: chromosome
• Accession: NZ_CP020107
• Organism: Escherichia coli strain 13E0767

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A2A3UTQ9
• Locus tag: EC767_RS21200
• Protein ID: WP_001094399.1
• Coordinates: 4079851..4080219 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EC767_RS21205
• Protein ID: WP_077249034.1
• Coordinates: 4080240..4080545 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC767_RS21180	4075024..4075980	+	957	WP_040092110.1	aldehyde oxidoreductase FAD-binding subunit PaoB	-
EC767_RS21185	4075977..4078175	+	2199	WP_000667026.1	aldehyde oxidoreductase molybdenum-binding subunit PaoC	-
EC767_RS21190	4078185..4079141	+	957	WP_000121330.1	molybdenum cofactor insertion chaperone PaoD	-
EC767_RS21195	4079192..4079530	+	339	Protein_3891	LysR family transcriptional regulator	-
EC767_RS21200	4079851..4080219	-	369	WP_001094399.1	TA system toxin CbtA family protein	Toxin
EC767_RS21205	4080240..4080545	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EC767_RS21210	4080649..4081293	-	645	WP_000086755.1	hypothetical protein	-
EC767_RS21215	4081312..4081533	-	222	WP_000692312.1	DUF987 domain-containing protein	-
EC767_RS21220	4081596..4082072	-	477	WP_021513354.1	RadC family protein	-
EC767_RS21225	4082088..4082561	-	474	WP_021552431.1	antirestriction protein	-
EC767_RS21235	4082862..4083680	-	819	WP_080029847.1	DUF945 domain-containing protein	-
EC767_RS21240	4083771..4084004	-	234	WP_001117568.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	4055453..4120760	65307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13568.73 Da
• Isoelectric Point: 7.7465

>T74149 WP_001094399.1 NZ_CP020107:c4080219-4079851 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T74149 NZ_CP020107:c4080219-4079851 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
ACCTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT74149 WP_077249034.1 NZ_CP020107:c4080545-4080240 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT74149 NZ_CP020107:c4080545-4080240 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A2A3UTQ9