Detailed information of TA system TA074145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2497424..2497950
• Replicon: chromosome
• Accession: NZ_CP020107
• Organism: Escherichia coli strain 13E0767

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: EC767_RS12670
• Protein ID: WP_000323025.1
• Coordinates: 2497663..2497950 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: EC767_RS12665
• Protein ID: WP_000534858.1
• Coordinates: 2497424..2497663 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC767_RS12630	2492758..2493114	+	357	WP_001374839.1	class I SAM-dependent methyltransferase	-
EC767_RS26130	2493725..2494012	+	288	Protein_2312	hypothetical protein	-
EC767_RS12650	2494781..2496122	+	1342	Protein_2313	ISNCY family transposase	-
EC767_RS12655	2496559..2496891	-	333	WP_001317460.1	FlxA-like family protein	-
EC767_RS12660	2497094..2497399	-	306	WP_001326990.1	hypothetical protein	-
EC767_RS12665	2497424..2497663	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
EC767_RS12670	2497663..2497950	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
EC767_RS12675	2498022..2498177	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
EC767_RS12680	2498394..2498645	+	252	WP_000980994.1	hypothetical protein	-
EC767_RS12685	2498712..2498990	+	279	WP_012304870.1	hypothetical protein	-
EC767_RS12690	2498992..2500041	+	1050	WP_001265198.1	DUF968 domain-containing protein	-
EC767_RS12695	2500055..2500807	+	753	WP_001047135.1	antitermination protein	-
EC767_RS25875	2501085..2501174	-	90	WP_120795389.1	hypothetical protein	-
EC767_RS12700	2501229..2501441	-	213	WP_000087756.1	cold shock-like protein CspF	-
EC767_RS12705	2501742..2501957	+	216	WP_000066484.1	cold shock-like protein CspB	-
EC767_RS26240	2502321..2502491	-	171	WP_211180497.1	putative zinc-binding protein YnfU	-
EC767_RS12710	2502711..2502926	+	216	WP_000839590.1	phage lysis protein EssD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2472525..2545927	73402

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T74145 WP_000323025.1 NZ_CP020107:2497663-2497950 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T74145 NZ_CP020107:2497663-2497950 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT74145 WP_000534858.1 NZ_CP020107:2497424-2497663 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT74145 NZ_CP020107:2497424-2497663 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8