Detailed information of TA system TA074135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1560716..1561341
• Replicon: chromosome
• Accession: NZ_CP020107
• Organism: Escherichia coli strain 13E0767

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EC767_RS07850
• Protein ID: WP_000911330.1
• Coordinates: 1560943..1561341 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EC767_RS07845
• Protein ID: WP_000450524.1
• Coordinates: 1560716..1560943 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC767_RS07820	1556519..1556989	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EC767_RS07825	1556989..1557561	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EC767_RS07830	1557707..1558585	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EC767_RS07835	1558602..1559636	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EC767_RS07840	1559849..1560562	+	714	WP_040092275.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EC767_RS07845	1560716..1560943	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EC767_RS07850	1560943..1561341	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EC767_RS07855	1561488..1562351	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
EC767_RS07860	1562366..1564381	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
EC767_RS07865	1564455..1565153	+	699	WP_000679812.1	esterase	-
EC767_RS07870	1565263..1565463	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T74135 WP_000911330.1 NZ_CP020107:1560943-1561341 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T74135 NZ_CP020107:1560943-1561341 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT74135 WP_000450524.1 NZ_CP020107:1560716-1560943 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT74135 NZ_CP020107:1560716-1560943 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33