Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1140588..1141242 Replicon chromosome
Accession NZ_CP020107
Organism Escherichia coli strain 13E0767

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EC767_RS05770 Protein ID WP_000244772.1
Coordinates 1140835..1141242 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EC767_RS05765 Protein ID WP_000354046.1
Coordinates 1140588..1140854 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC767_RS05740 1135757..1136500 + 744 WP_000951964.1 SDR family oxidoreductase -
EC767_RS05745 1136557..1137990 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
EC767_RS05750 1138035..1138346 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EC767_RS05755 1138510..1139169 + 660 WP_000250274.1 hemolysin III family protein -
EC767_RS05760 1139365..1140345 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EC767_RS05765 1140588..1140854 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EC767_RS05770 1140835..1141242 + 408 WP_000244772.1 protein YgfX Toxin
EC767_RS05775 1141282..1141803 - 522 WP_001055874.1 flavodoxin FldB -
EC767_RS05780 1141915..1142811 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EC767_RS05785 1142836..1143546 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EC767_RS05790 1143552..1145285 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T74132 WP_000244772.1 NZ_CP020107:1140835-1141242 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T74132 NZ_CP020107:1140835-1141242 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT74132 WP_000354046.1 NZ_CP020107:1140588-1140854 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74132 NZ_CP020107:1140588-1140854 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References