Detailed information of TA system TA074122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2900972..2901610
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EC780_RS15580
• Protein ID: WP_000813794.1
• Coordinates: 2901434..2901610 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EC780_RS15575
• Protein ID: WP_001270286.1
• Coordinates: 2900972..2901388 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS15555	2896124..2897065	-	942	WP_032314702.1	ABC transporter permease	-
EC780_RS15560	2897066..2898079	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
EC780_RS15565	2898097..2899242	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
EC780_RS15570	2899487..2900893	-	1407	WP_000760615.1	PLP-dependent aminotransferase family protein	-
EC780_RS15575	2900972..2901388	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EC780_RS15580	2901434..2901610	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EC780_RS15585	2901832..2902062	+	231	WP_000494244.1	YncJ family protein	-
EC780_RS15590	2902154..2904115	-	1962	WP_032314701.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EC780_RS15595	2904188..2904724	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
EC780_RS15600	2904777..2905991	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2906031..2907179	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T74122 WP_000813794.1 NZ_CP020106:c2901610-2901434 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T74122 NZ_CP020106:c2901610-2901434 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT74122 WP_001270286.1 NZ_CP020106:c2901388-2900972 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT74122 NZ_CP020106:c2901388-2900972 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB