Detailed information of TA system TA074107

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1644552..1645177
• Replicon: chromosome
• Accession: NZ_CP020106
• Organism: Escherichia coli strain 13E0780

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EC780_RS08815
• Protein ID: WP_000911330.1
• Coordinates: 1644779..1645177 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: EC780_RS08810
• Protein ID: WP_000450524.1
• Coordinates: 1644552..1644779 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC780_RS08785	1640355..1640825	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
EC780_RS08790	1640825..1641397	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
EC780_RS08795	1641543..1642421	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
EC780_RS08800	1642438..1643472	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
EC780_RS08805	1643685..1644398	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
EC780_RS08810	1644552..1644779	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
EC780_RS08815	1644779..1645177	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
EC780_RS08820	1645324..1646187	+	864	WP_080029410.1	neutral zinc metallopeptidase	-
EC780_RS08825	1646202..1648217	+	2016	WP_032314290.1	tRNA cytosine(34) acetyltransferase TmcA	-
EC780_RS08830	1648291..1648989	+	699	WP_000679823.1	esterase	-
EC780_RS08835	1649099..1649299	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T74107 WP_000911330.1 NZ_CP020106:1644779-1645177 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T74107 NZ_CP020106:1644779-1645177 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT74107 WP_000450524.1 NZ_CP020106:1644552-1644779 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT74107 NZ_CP020106:1644552-1644779 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33