Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1134166..1134820 Replicon chromosome
Accession NZ_CP020106
Organism Escherichia coli strain 13E0780

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EC780_RS06120 Protein ID WP_000244777.1
Coordinates 1134413..1134820 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EC780_RS06115 Protein ID WP_000354046.1
Coordinates 1134166..1134432 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC780_RS06090 1129335..1130078 + 744 WP_000951964.1 SDR family oxidoreductase -
EC780_RS06095 1130135..1131568 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
EC780_RS06100 1131613..1131924 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
EC780_RS06105 1132088..1132747 + 660 WP_032314344.1 hemolysin III family protein -
EC780_RS06110 1132943..1133923 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EC780_RS06115 1134166..1134432 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EC780_RS06120 1134413..1134820 + 408 WP_000244777.1 toxin CptA Toxin
EC780_RS06125 1134860..1135381 - 522 WP_001055874.1 flavodoxin FldB -
EC780_RS06130 1135493..1136389 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EC780_RS06135 1136414..1137124 + 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EC780_RS06140 1137130..1138863 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T74104 WP_000244777.1 NZ_CP020106:1134413-1134820 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T74104 NZ_CP020106:1134413-1134820 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT74104 WP_000354046.1 NZ_CP020106:1134166-1134432 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74104 NZ_CP020106:1134166-1134432 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References