Detailed information of TA system TA074074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3335210..3336861
• Replicon: chromosome
• Accession: NZ_CP020100
• Organism: Pseudomonas sp. S-6-2

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: BVH74_RS15460
• Protein ID: WP_080050960.1
• Coordinates: 3335210..3336517 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: BVH74_RS15465
• Protein ID: WP_080050962.1
• Coordinates: 3336514..3336861 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BVH74_RS15445	3330322..3333357	-	3036	WP_080050955.1	efflux RND transporter permease subunit	-
BVH74_RS15450	3333354..3334457	-	1104	WP_080050957.1	efflux RND transporter periplasmic adaptor subunit	-
BVH74_RS15455	3334567..3335202	+	636	WP_080050958.1	TetR/AcrR family transcriptional regulator	-
BVH74_RS15460	3335210..3336517	-	1308	WP_080050960.1	type II toxin-antitoxin system HipA family toxin	Toxin
BVH74_RS15465	3336514..3336861	-	348	WP_080050962.1	helix-turn-helix transcriptional regulator	Antitoxin
BVH74_RS15470	3337028..3337471	-	444	WP_080050963.1	DoxX family protein	-
BVH74_RS15475	3337564..3338838	-	1275	WP_080050965.1	D-serine ammonia-lyase	-
BVH74_RS15480	3338904..3339086	+	183	WP_080051759.1	DUF2897 family protein	-
BVH74_RS15485	3339134..3339658	+	525	WP_080050967.1	DUF2058 domain-containing protein	-
BVH74_RS15490	3339698..3340372	-	675	WP_080050969.1	RimK/LysX family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (170-408)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-67)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 48420.06 Da
• Isoelectric Point: 7.4009

>T74074 WP_080050960.1 NZ_CP020100:c3336517-3335210 [Pseudomonas sp. S-6-2]
MTTLAEVRLWGRTIGAVAQEPGEATAAFQYDPGFIGSGIELSPLVMPLSPRVYRFPELPRQTFHGLPGLLADCLPDKFGN
ALIDAWLATQGRSPDSFNAVERLCYTGRRGMGALEFLPTTGPQARESERVSIDALVELASEVLAHRNDLKGSFADADKAA
ALRDILRVGTSAGGARAKAVIAWQPQTNEVRSGQVEAGEGFEYWLLKFDGVSGNRDKELDDPQGYCAIEYAYYLMAREAG
IDMSECRLLEEGGRRHFMTRRFDRLPGGGKLHMQSLCALAHFDFNQAGAYSYEQALQVIRRLGLPMAALEQQFRRMLFNI
VARNQDDHVKNIAFLMDRQGRWSLSPAFDVTYSYNPEGMWTASHQMSLNGKRDHFTLDDIKACARVAGLKRGRAESLLRE
VAEVVSQWPAFADRAAVAPRWRDQITRTLRLKLAG

Nucleotide

• Length: 1308 bp

>T74074 NZ_CP020100:c3336517-3335210 [Pseudomonas sp. S-6-2]
ATGACCACACTGGCCGAGGTCCGGCTATGGGGGCGGACCATTGGCGCGGTAGCACAGGAGCCGGGTGAAGCTACGGCGGC
GTTCCAGTACGATCCGGGGTTTATTGGTAGCGGCATTGAACTGTCGCCATTGGTGATGCCCTTGTCACCACGGGTTTACC
GCTTTCCTGAGCTGCCCAGGCAAACTTTTCACGGTTTGCCTGGGCTGCTGGCTGACTGTTTGCCGGACAAATTCGGCAAT
GCTCTGATCGATGCCTGGCTGGCAACTCAGGGGCGTAGCCCGGACAGTTTCAATGCGGTAGAGCGCCTGTGTTACACCGG
GCGGCGGGGGATGGGGGCGCTTGAGTTTTTGCCGACTACTGGACCTCAGGCCCGTGAGTCGGAGCGGGTCAGCATCGATG
CGTTGGTTGAGTTGGCCTCTGAAGTGCTGGCGCACCGTAACGATCTGAAGGGCAGTTTTGCTGATGCCGACAAGGCTGCG
GCCCTGCGTGACATTCTGCGAGTGGGAACCTCGGCCGGTGGCGCCCGAGCCAAAGCTGTGATTGCCTGGCAGCCGCAGAC
CAATGAGGTACGTTCTGGTCAGGTCGAGGCTGGAGAAGGTTTTGAATACTGGCTACTGAAGTTCGATGGGGTTTCCGGCA
ATCGCGACAAGGAGCTGGATGATCCTCAGGGTTATTGTGCGATCGAATATGCCTACTACCTGATGGCCCGCGAGGCGGGT
ATTGACATGAGTGAGTGCCGGTTGCTGGAGGAAGGCGGGCGCCGGCACTTCATGACCCGGCGGTTTGATCGGTTGCCCGG
CGGCGGCAAGTTGCACATGCAGTCACTGTGTGCGCTGGCTCATTTCGACTTCAATCAGGCTGGTGCCTACAGCTACGAGC
AGGCGTTGCAGGTGATTCGCCGCCTGGGGCTGCCGATGGCGGCGCTGGAGCAGCAATTCCGGCGCATGCTGTTCAATATC
GTCGCCCGTAACCAGGATGATCACGTCAAGAACATTGCCTTTCTGATGGATCGTCAGGGGCGCTGGTCATTATCGCCGGC
CTTTGACGTGACCTACAGCTACAATCCCGAGGGTATGTGGACCGCGAGTCATCAGATGAGCCTCAATGGCAAGCGCGATC
ACTTCACCCTGGATGATATCAAGGCCTGTGCCCGGGTTGCCGGCCTAAAGCGCGGACGCGCTGAATCTCTGTTGCGGGAG
GTGGCCGAGGTGGTTAGTCAATGGCCGGCGTTTGCTGACCGGGCTGCCGTGGCGCCGCGCTGGCGCGATCAGATTACCCG
AACCCTACGCCTGAAGCTGGCTGGTTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12379.19 Da
• Isoelectric Point: 10.2778

>AT74074 WP_080050962.1 NZ_CP020100:c3336861-3336514 [Pseudomonas sp. S-6-2]
MRLSDSLTDDAVLAELGGRLARLRLDSGLTQAQLAEQAGLSKRTLERLEAGQSSQLLSLIRVLRVLGRLGGLEALLPDTG
PGPMALLRGKGKPPQRASRRAVAEPKGEWQWGDDE

Nucleotide

• Length: 348 bp

>AT74074 NZ_CP020100:c3336861-3336514 [Pseudomonas sp. S-6-2]
ATGCGGTTATCTGACTCGCTGACTGATGATGCAGTGTTGGCCGAGCTGGGAGGGCGGTTGGCCCGGCTGCGACTTGACAG
TGGTTTGACCCAGGCCCAGTTGGCGGAGCAGGCTGGTTTGTCCAAGCGTACTCTGGAGCGTCTGGAGGCGGGGCAGTCCA
GCCAACTGCTCAGTCTGATTCGGGTGCTGCGGGTGTTGGGTCGGCTGGGCGGCCTGGAAGCCTTGTTGCCGGATACCGGA
CCGGGGCCAATGGCGCTGCTACGTGGCAAGGGCAAGCCGCCGCAACGAGCATCGCGCCGTGCGGTGGCCGAGCCCAAGGG
CGAATGGCAGTGGGGAGATGACGAATGA