Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3906691..3907309 Replicon chromosome
Accession NZ_CP020092
Organism Escherichia coli strain 13E0725

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EC725_RS20855 Protein ID WP_001291435.1
Coordinates 3907091..3907309 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EC725_RS20850 Protein ID WP_000344800.1
Coordinates 3906691..3907065 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC725_RS20840 3901780..3902973 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EC725_RS20845 3902996..3906145 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EC725_RS20850 3906691..3907065 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EC725_RS20855 3907091..3907309 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EC725_RS20860 3907480..3908031 + 552 WP_032314633.1 maltose O-acetyltransferase -
EC725_RS20865 3908147..3908617 + 471 WP_000136192.1 YlaC family protein -
EC725_RS20870 3908781..3910331 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EC725_RS20875 3910373..3910726 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EC725_RS20885 3911105..3911416 + 312 WP_000409911.1 MGMT family protein -
EC725_RS20890 3911447..3912019 - 573 WP_000779826.1 lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T74067 WP_001291435.1 NZ_CP020092:3907091-3907309 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T74067 NZ_CP020092:3907091-3907309 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT74067 WP_000344800.1 NZ_CP020092:3906691-3907065 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT74067 NZ_CP020092:3906691-3907065 [Escherichia coli]
ATGGATGAATACTCACCTAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References