Detailed information of TA system TA074066

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2742195..2742833
• Replicon: chromosome
• Accession: NZ_CP020092
• Organism: Escherichia coli strain 13E0725

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: EC725_RS14400
• Protein ID: WP_000813794.1
• Coordinates: 2742657..2742833 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EC725_RS14395
• Protein ID: WP_001270286.1
• Coordinates: 2742195..2742611 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EC725_RS14375	2737347..2738288	-	942	WP_032314702.1	ABC transporter permease	-
EC725_RS14380	2738289..2739302	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
EC725_RS14385	2739320..2740465	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
EC725_RS14390	2740710..2742116	-	1407	WP_000760615.1	PLP-dependent aminotransferase family protein	-
EC725_RS14395	2742195..2742611	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EC725_RS14400	2742657..2742833	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EC725_RS14405	2743055..2743285	+	231	WP_000494244.1	YncJ family protein	-
EC725_RS14410	2743377..2745338	-	1962	WP_032314701.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EC725_RS14415	2745411..2745947	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
EC725_RS14420	2746000..2747214	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2747254..2748402	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T74066 WP_000813794.1 NZ_CP020092:c2742833-2742657 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T74066 NZ_CP020092:c2742833-2742657 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT74066 WP_001270286.1 NZ_CP020092:c2742611-2742195 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT74066 NZ_CP020092:c2742611-2742195 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB