Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 842588..843386 Replicon chromosome
Accession NZ_CP020092
Organism Escherichia coli strain 13E0725

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0F3UPG9
Locus tag EC725_RS04425 Protein ID WP_000854698.1
Coordinates 843009..843386 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0F3T7G0
Locus tag EC725_RS04420 Protein ID WP_000017094.1
Coordinates 842588..842962 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC725_RS27475 839470..839925 + 456 Protein_784 hypothetical protein -
EC725_RS27480 840044..840255 - 212 Protein_785 hypothetical protein -
EC725_RS04400 840336..841154 + 819 WP_001234402.1 DUF945 domain-containing protein -
EC725_RS04405 841246..841731 + 486 WP_000213709.1 antirestriction protein -
EC725_RS04410 841747..842223 + 477 WP_001186714.1 RadC family protein -
EC725_RS04415 842292..842513 + 222 WP_001220316.1 DUF987 domain-containing protein -
EC725_RS04420 842588..842962 + 375 WP_000017094.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EC725_RS04425 843009..843386 + 378 WP_000854698.1 TA system toxin CbtA family protein Toxin
EC725_RS04430 843383..843871 + 489 WP_001054226.1 hypothetical protein -
EC725_RS04435 843883..844080 + 198 WP_000839251.1 DUF957 domain-containing protein -
EC725_RS04440 844165..844491 + 327 WP_033815866.1 hypothetical protein -
EC725_RS04445 844525..846063 - 1539 WP_000998077.1 IS66-like element ISEc8 family transposase -
EC725_RS04450 846113..846460 - 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
EC725_RS04455 846457..846837 - 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
EC725_RS04460 846930..847457 + 528 Protein_798 DUF4942 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 841747..856793 15046


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13975.94 Da        Isoelectric Point: 7.9087

>T74051 WP_000854698.1 NZ_CP020092:843009-843386 [Escherichia coli]
MKTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRSHYRTVNDITLGKRTEAKR

Download         Length: 378 bp

>T74051 NZ_CP020092:843009-843386 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CCACTACAGAACGGTAAATGACATTACCCTGGGTAAACGCACGGAGGCAAAACGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13732.42 Da        Isoelectric Point: 5.4488

>AT74051 WP_000017094.1 NZ_CP020092:842588-842962 [Escherichia coli]
VSGTLHETNYPNDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT74051 NZ_CP020092:842588-842962 [Escherichia coli]
GTGTCAGGCACACTCCATGAGACAAATTATCCCAACGACAACAACGACCGCCCCTGGTGGGGGCTCCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAATTACATTACCTTGCCGACCGCGCCGGTATCAGGGGGCAGT
TTAGCGACGCGGATGCGTATCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UPG9

Antitoxin

Source ID Structure
AlphaFold DB A0A0F3T7G0

References