Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4446819..4447498 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name ykfI Uniprot ID A0A8H9RR71
Locus tag B2J95_RS21875 Protein ID WP_032655083.1
Coordinates 4446819..4447160 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A8H9RPW5
Locus tag B2J95_RS21880 Protein ID WP_001548170.1
Coordinates 4447181..4447498 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS21855 4442088..4443833 + 1746 WP_014833324.1 DNA primase -
B2J95_RS21860 4443999..4445846 + 1848 WP_013098777.1 RNA polymerase sigma factor RpoD -
B2J95_RS21865 4445950..4446456 - 507 WP_020689452.1 G/U mismatch-specific DNA glycosylase -
B2J95_RS21875 4446819..4447160 - 342 WP_032655083.1 TA system toxin CbtA family protein Toxin
B2J95_RS21880 4447181..4447498 - 318 WP_001548170.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
B2J95_RS21885 4447518..4447739 - 222 WP_032655087.1 DUF987 domain-containing protein -
B2J95_RS21890 4447748..4448224 - 477 WP_032655090.1 RadC family protein -
B2J95_RS21895 4448240..4448698 - 459 WP_032655093.1 antirestriction protein -
B2J95_RS21900 4448800..4449039 - 240 WP_001115184.1 DUF905 domain-containing protein -
B2J95_RS21905 4449130..4450890 - 1761 Protein_4163 autotransporter domain-containing protein -
B2J95_RS21910 4451204..4452031 - 828 WP_000197390.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13031.15 Da        Isoelectric Point: 10.4476

>T74045 WP_032655083.1 NZ_CP020089:c4447160-4446819 [Enterobacter cloacae]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRKNLVR

Download         Length: 342 bp

>T74045 NZ_CP020089:c4447160-4446819 [Enterobacter cloacae]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTACTGAC
CCGCCTGCTGAAACAGCACTATGGTCTCACCCTCAACGACACACCGTTCAGTGAGGAACGTGTTATACAGGAACATATCG
ATGCCGGAATCACGCTGGCTGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAAAAATTTGGTACGGTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11883.46 Da        Isoelectric Point: 7.0810

>AT74045 WP_001548170.1 NZ_CP020089:c4447498-4447181 [Enterobacter cloacae]
MTHINWGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT74045 NZ_CP020089:c4447498-4447181 [Enterobacter cloacae]
ATGACCCACATCAACTGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCATATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References