Detailed information of TA system TA074044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 4293927..4294708
• Replicon: chromosome
• Accession: NZ_CP020089
• Organism: Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: B2J95_RS21130
• Protein ID: WP_058660963.1
• Coordinates: 4293927..4294418 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A5E1AUV7
• Locus tag: B2J95_RS21135
• Protein ID: WP_029882966.1
• Coordinates: 4294415..4294708 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2J95_RS21110	4289711..4290403	+	693	WP_058678478.1	response regulator	-
B2J95_RS21115	4290458..4292593	-	2136	WP_058678477.1	ornithine decarboxylase	-
B2J95_RS21120	4292776..4293489	+	714	WP_058678476.1	DUF554 domain-containing protein	-
B2J95_RS21130	4293927..4294418	-	492	WP_058660963.1	GNAT family N-acetyltransferase	Toxin
B2J95_RS21135	4294415..4294708	-	294	WP_029882966.1	DUF1778 domain-containing protein	Antitoxin
B2J95_RS21140	4294957..4296744	-	1788	Protein_4020	hybrid sensor histidine kinase/response regulator	-
B2J95_RS21145	4296741..4297373	-	633	WP_013098693.1	response regulator transcription factor	-
B2J95_RS21150	4297549..4297863	+	315	WP_013098694.1	lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (1-95)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17647.56 Da
• Isoelectric Point: 7.2653

>T74044 WP_058660963.1 NZ_CP020089:c4294418-4293927 [Enterobacter cloacae]
MISAPEPLHAGHMLTPFCCGVDSMDNWLKQRAMKNQVSGASRTFVCCGNDSNVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSLHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMILMVTLGDLM
ASI

Nucleotide

• Length: 492 bp

>T74044 NZ_CP020089:c4294418-4293927 [Enterobacter cloacae]
ATGATCTCTGCCCCAGAGCCCCTTCACGCCGGTCATATGCTTACTCCATTTTGCTGTGGTGTGGATTCGATGGATAACTG
GCTGAAGCAGCGGGCGATGAAAAATCAGGTCAGTGGTGCATCCCGTACCTTTGTCTGTTGTGGCAACGATTCTAACGTTC
TGGCTTATTACTCGCTGGCATCCAGTGCGGTCATGACGAATACCGCACCCGGTCGCTTTCGTCGCAATATGCCCGATCCC
ATCCCGGTTGTTGTGCTGGGACGCCTGGCGGTAGATCAGTCTCTGCATGGGCAGGGCGTTGGTCGGGCACTGGTACGCGA
CGCCGGGCTGCGGGTGATCCAGGTGGCGGAGACCATCGGCATTCGCGGAATGCTGGTTCATGCGCTGTCGGATGAAGCGC
GGGAATTTTATCTGCGGGTGGGGTTTGAGCCATCGCCAATGGATCCGATGATATTGATGGTGACGTTGGGGGATTTGATG
GCGAGTATTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11003.66 Da
• Isoelectric Point: 9.9156

>AT74044 WP_029882966.1 NZ_CP020089:c4294708-4294415 [Enterobacter cloacae]
MPAANTMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLARLDQAPAPN
ATLRKTMQTPAPWEREK

Nucleotide

• Length: 294 bp

>AT74044 NZ_CP020089:c4294708-4294415 [Enterobacter cloacae]
ATGCCAGCTGCAAACACTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAGGCCTGCCGAGCGAGATCTTATCGA
TCGGGCGGCGAAAGCGAGAGGGAAAAACCGCACTGACTTTGTGCTGGAAGCAGCCCGCGCTGCTGCGGAAGAGGCACTGA
TCGAACAACGCATCATAATGGCCGATCCAGAGGCTTATCAGGAATTTCTGGCTCGTCTGGATCAGGCTCCAGCACCTAAT
GCCACACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCGGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5E1AUV7