Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4200789..4201449 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name cptA Uniprot ID A0A5E1AGQ2
Locus tag B2J95_RS20655 Protein ID WP_023620447.1
Coordinates 4200789..4201202 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A5E1AG01
Locus tag B2J95_RS20660 Protein ID WP_013098615.1
Coordinates 4201183..4201449 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS20635 4196782..4198515 - 1734 WP_058679192.1 single-stranded-DNA-specific exonuclease RecJ -
B2J95_RS20640 4198521..4199234 - 714 WP_013098611.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
B2J95_RS20645 4199263..4200159 - 897 WP_013098612.1 site-specific tyrosine recombinase XerD -
B2J95_RS20650 4200261..4200782 + 522 WP_013098613.1 flavodoxin FldB -
B2J95_RS20655 4200789..4201202 - 414 WP_023620447.1 protein YgfX Toxin
B2J95_RS20660 4201183..4201449 - 267 WP_013098615.1 FAD assembly factor SdhE Antitoxin
B2J95_RS20665 4201744..4202724 + 981 WP_023620448.1 tRNA-modifying protein YgfZ -
B2J95_RS20670 4202834..4203493 - 660 WP_003863433.1 hemolysin III family protein -
B2J95_RS20675 4203759..4204490 + 732 WP_058679193.1 MurR/RpiR family transcriptional regulator -
B2J95_RS20680 4204607..4206040 + 1434 WP_058679194.1 6-phospho-beta-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-132)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16220.21 Da        Isoelectric Point: 10.9554

>T74043 WP_023620447.1 NZ_CP020089:c4201202-4200789 [Enterobacter cloacae]
VVLWQSDLRVSWRSQWMSLLLHGLVAAFVLLMPWPLSYTPLWLLLLSFVVFDSVRSQRRINARQGEIKLLMDSRLRWQGK
EWDILGMPWMLSSGMMLRLRQVGGGKCQHLWLAADSMDIAEWRELRRMLLQQQPTQE

Download         Length: 414 bp

>T74043 NZ_CP020089:c4201202-4200789 [Enterobacter cloacae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCGCAGTGGATGTCGTTATTGCTCCACGGCCTGGTCGCGGC
ATTTGTTTTACTGATGCCGTGGCCGCTTAGCTATACGCCGCTGTGGCTGTTGTTGCTGTCGTTCGTCGTTTTTGACAGCG
TGCGCAGCCAGCGCCGGATCAATGCCCGCCAGGGTGAGATCAAATTACTGATGGACTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATCCTCGGTATGCCCTGGATGCTCAGTTCCGGCATGATGCTACGGTTACGCCAGGTGGGCGGCGGTAAGTG
CCAGCATCTGTGGCTGGCCGCCGACAGTATGGATATTGCGGAGTGGCGGGAACTGCGCCGGATGCTACTGCAGCAGCAAC
CGACGCAGGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10569.05 Da        Isoelectric Point: 5.1456

>AT74043 WP_013098615.1 NZ_CP020089:c4201449-4201183 [Enterobacter cloacae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDSLSDEDKRLFVRLLESDDPDLFNWLMNHGKPADTELQRMVQLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT74043 NZ_CP020089:c4201449-4201183 [Enterobacter cloacae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCATGCCGACGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGAGGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCCGATT
TATTCAACTGGCTGATGAACCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AGQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AG01

References