Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3118817..3119372 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name shpA Uniprot ID A0A839BSP9
Locus tag B2J95_RS15155 Protein ID WP_071881499.1
Coordinates 3119106..3119372 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A839BUZ1
Locus tag B2J95_RS15150 Protein ID WP_038416295.1
Coordinates 3118817..3119119 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS15120 3114032..3115107 + 1076 Protein_2888 IS110 family transposase -
B2J95_RS15125 3115124..3115411 + 288 Protein_2889 DDE-type integrase/transposase/recombinase -
B2J95_RS15130 3115443..3116146 + 704 Protein_2890 IS5 family transposase -
B2J95_RS15140 3116445..3117356 + 912 WP_058679564.1 acetamidase/formamidase family protein -
B2J95_RS15145 3117353..3118696 + 1344 WP_038416294.1 APC family permease -
B2J95_RS15150 3118817..3119119 - 303 WP_038416295.1 BrnA antitoxin family protein Antitoxin
B2J95_RS15155 3119106..3119372 - 267 WP_071881499.1 BrnT family toxin Toxin
B2J95_RS15165 3119381..3119728 + 348 Protein_2895 transposase -
B2J95_RS15170 3119701..3119775 - 75 Protein_2896 hypothetical protein -
B2J95_RS15175 3119862..3120617 - 756 WP_058679565.1 M48 family metallopeptidase -
B2J95_RS15180 3120614..3123898 - 3285 WP_058679566.1 type I restriction endonuclease subunit R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3110961..3142329 31368
- inside IScluster/Tn - - 3110629..3119815 9186


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(9-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10474.91 Da        Isoelectric Point: 10.0832

>T74041 WP_071881499.1 NZ_CP020089:c3119372-3119106 [Enterobacter cloacae]
IRDLFAPSLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLSLVHRIIVIMVAHSVRFESGTEVIRIISARKADSK
ERNRYEHG

Download         Length: 267 bp

>T74041 NZ_CP020089:c3119372-3119106 [Enterobacter cloacae]
ATCAGGGACTTGTTCGCACCTTCCCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGTGCTCGTCTTTGACGATCCCCG
GCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCAGCCTCGTTCATCGGATCATTGTTA
TCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGCGCCCGGAAGGCGGACAGCAAA
GAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11637.21 Da        Isoelectric Point: 9.4429

>AT74041 WP_038416295.1 NZ_CP020089:c3119119-3118817 [Enterobacter cloacae]
MNMVKHKRDNPDTLTTKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT74041 NZ_CP020089:c3119119-3118817 [Enterobacter cloacae]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGACTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BSP9

Antitoxin

Source ID Structure
AlphaFold DB A0A839BUZ1

References