Detailed information of TA system TA074032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1730547..1731460
• Replicon: chromosome
• Accession: NZ_CP020089
• Organism: Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: B2J95_RS08435
• Protein ID: WP_058679276.1
• Coordinates: 1730547..1731020 (-)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: B2J95_RS08440
• Protein ID: WP_038981400.1
• Coordinates: 1731017..1731460 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2J95_RS08415	1726705..1728015	+	1311	WP_058679280.1	type I-F CRISPR-associated protein Csy1	-
B2J95_RS08420	1728012..1728947	+	936	WP_058679279.1	type I-F CRISPR-associated protein Csy2	-
B2J95_RS08425	1728961..1729959	+	999	WP_058679278.1	type I-F CRISPR-associated protein Csy3	-
B2J95_RS08430	1729970..1730524	+	555	WP_058679277.1	type I-F CRISPR-associated endoribonuclease Cas6/Csy4	-
B2J95_RS08435	1730547..1731020	-	474	WP_058679276.1	RES domain-containing protein	Toxin
B2J95_RS08440	1731017..1731460	-	444	WP_038981400.1	DUF2384 domain-containing protein	Antitoxin
B2J95_RS08445	1731765..1731983	-	219	WP_002211347.1	translation initiation factor IF-1	-
B2J95_RS08450	1732268..1732972	-	705	WP_013097324.1	leucyl/phenylalanyl-tRNA--protein transferase	-
B2J95_RS08455	1733018..1734739	-	1722	WP_058679275.1	cysteine/glutathione ABC transporter ATP-binding protein/permease CydC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (94-144)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 17721.99 Da
• Isoelectric Point: 4.7156

>T74032 WP_058679276.1 NZ_CP020089:c1731020-1730547 [Enterobacter cloacae]
MIFYRLVTGRYASEAWSGGGANQYGGRWNHKGHPAVYVSTSISLASLEILVHVKKDTVLNQYQLFSIDIPEEQIEYLDKA
WLPEDWQEDPAPMSTMDLGTGWLQSSSALALILPSCIIPYENNAILNPLHPDFHQRLETVQQLPFSFDNRLAEKTAS

Nucleotide

• Length: 474 bp

>T74032 NZ_CP020089:c1731020-1730547 [Enterobacter cloacae]
ATGATTTTTTATCGTCTGGTGACAGGACGATATGCCAGCGAAGCGTGGAGTGGAGGCGGCGCAAACCAGTATGGCGGCCG
CTGGAATCACAAAGGACACCCGGCGGTATACGTCTCTACATCCATCTCGCTGGCATCACTTGAAATTCTGGTTCACGTTA
AAAAAGATACCGTGTTAAACCAATACCAGCTTTTCAGCATTGATATCCCGGAAGAGCAAATTGAGTATCTGGATAAAGCA
TGGCTACCCGAAGACTGGCAGGAGGATCCTGCACCGATGTCGACAATGGATCTGGGAACCGGTTGGCTCCAGTCCAGCAG
CGCGCTGGCGCTTATTCTACCGTCCTGCATCATTCCTTATGAAAATAACGCCATACTTAATCCACTGCATCCCGATTTTC
ATCAGAGACTGGAGACCGTCCAGCAACTCCCTTTTAGCTTTGATAACCGGCTAGCAGAAAAAACCGCATCCTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16234.50 Da
• Isoelectric Point: 6.4772

>AT74032 WP_038981400.1 NZ_CP020089:c1731460-1731017 [Enterobacter cloacae]
MRTWVPAQKPADNALWRYAGLPANRGMQLIELLSQGLPVSVLDNIHEWTAMSKADILRVTGINERNVARRKSAGRTLTPD
ESERVARFVRVLDAAVDYFGSKDDAWNWLQSPVRGLGNVAPVDLIATETGALEVTDLIGRLEHGVFA

Nucleotide

• Length: 444 bp

>AT74032 NZ_CP020089:c1731460-1731017 [Enterobacter cloacae]
ATGAGAACATGGGTTCCTGCGCAGAAGCCTGCTGATAACGCACTTTGGCGCTATGCCGGTTTACCTGCAAATCGAGGTAT
GCAGCTGATTGAGCTTCTTAGTCAGGGTCTACCCGTTAGCGTACTCGACAACATTCATGAATGGACGGCGATGTCAAAAG
CAGACATTTTGCGCGTCACCGGGATAAACGAGCGCAATGTCGCGCGTCGTAAAAGTGCGGGTCGTACATTGACCCCCGAT
GAAAGTGAGCGCGTGGCGCGCTTCGTACGCGTTCTGGATGCTGCCGTTGATTATTTTGGCAGTAAAGATGATGCATGGAA
CTGGCTACAATCACCAGTCCGTGGACTCGGAAATGTTGCGCCTGTCGATTTGATTGCAACAGAAACCGGTGCACTGGAAG
TGACCGATTTGATTGGTCGCCTGGAACATGGAGTATTCGCATGA