Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1221320..1221940 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag B2J95_RS05950 Protein ID WP_013095889.1
Coordinates 1221320..1221538 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag B2J95_RS05955 Protein ID WP_008499288.1
Coordinates 1221566..1221940 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS05920 1217332..1217592 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
B2J95_RS05925 1217595..1217735 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
B2J95_RS05930 1217732..1218442 - 711 WP_023620096.1 GNAT family N-acetyltransferase -
B2J95_RS05935 1218544..1220004 + 1461 WP_058679164.1 PLP-dependent aminotransferase family protein -
B2J95_RS05940 1219976..1220443 - 468 WP_058679163.1 hypothetical protein -
B2J95_RS05945 1220559..1221110 - 552 WP_013095888.1 maltose O-acetyltransferase -
B2J95_RS05950 1221320..1221538 - 219 WP_013095889.1 hemolysin expression modulator Hha Toxin
B2J95_RS05955 1221566..1221940 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
B2J95_RS05960 1222453..1225599 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit -
B2J95_RS05965 1225622..1226815 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T74031 WP_013095889.1 NZ_CP020089:c1221538-1221320 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T74031 NZ_CP020089:c1221538-1221320 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAACCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACACTCGAACGCGT
GATTGAAAAAAATAAATACGAGCTTTCTGATAATGAACTGACCGTATTTTATTCAGCCGCTGACCATCGTCTTGCCGAGC
TGACCATGAATAAACTGTATGACAAAATCCCGCCTTCCGTGTGGAAATTTGTCCGTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT74031 WP_008499288.1 NZ_CP020089:c1221940-1221566 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT74031 NZ_CP020089:c1221940-1221566 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAATCATGGCTGGGTGAACGATCCAACATCGGCCATCAATTTGCAATTGAATGAGTTGATAGAGC
ATATCGCGACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAACTGATTGAGCAAATTGACGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCGCAGGATTTGCAGAAATGGCGTAAATCTGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References