Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 692376..693170 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name KacT Uniprot ID A0A7V7JFS7
Locus tag B2J95_RS03465 Protein ID WP_013095427.1
Coordinates 692376..692897 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A5E1AP76
Locus tag B2J95_RS03470 Protein ID WP_013095428.1
Coordinates 692901..693170 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS03440 687703..687864 + 162 WP_013095422.1 DUF1127 domain-containing protein -
B2J95_RS03445 687951..689339 - 1389 WP_058678942.1 PLP-dependent aminotransferase family protein -
B2J95_RS03450 689442..689921 + 480 WP_013095424.1 carboxymuconolactone decarboxylase family protein -
B2J95_RS03455 689908..690810 - 903 WP_023620661.1 LysR family transcriptional regulator -
B2J95_RS03460 690911..692326 + 1416 WP_058678941.1 aldehyde dehydrogenase family protein -
B2J95_RS03465 692376..692897 - 522 WP_013095427.1 GNAT family N-acetyltransferase Toxin
B2J95_RS03470 692901..693170 - 270 WP_013095428.1 DUF1778 domain-containing protein Antitoxin
B2J95_RS03475 693385..693708 + 324 WP_023620659.1 antibiotic biosynthesis monooxygenase -
B2J95_RS03480 693701..694099 + 399 WP_058678940.1 hypothetical protein -
B2J95_RS03485 694071..694766 + 696 WP_045295461.1 winged helix-turn-helix domain-containing protein -
B2J95_RS03495 694970..695440 + 471 WP_013095432.1 MarR family transcriptional regulator -
B2J95_RS03500 695437..696504 + 1068 WP_095440265.1 HlyD family secretion protein -
B2J95_RS03505 696494..697570 + 1077 WP_013095434.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element fosA - 654428..695052 40624


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19410.33 Da        Isoelectric Point: 9.2434

>T74030 WP_013095427.1 NZ_CP020089:c692897-692376 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Download         Length: 522 bp

>T74030 NZ_CP020089:c692897-692376 [Enterobacter cloacae]
GTGAATAACATGAAAATAGGGATTTTTTCAGATGACGTTGAGTATGATCTAAGCCATTTCGATTGCGGAGAAGAGAGCCT
AAACACCTTTCTGACGGCGCACCTGAAACGCCAGCATCGTGGGAAATTTTTGCGGGGTTACGTTCTTGTCGCATCCGGGG
AAAAGCCGCGGGTGTTGGGTTATTACACGCTGTCCGGGAGCTGCTTTGAAAAGGCGTACCTTCCTTCTAAAACCCAGCAG
AAGCGAGTGCCTTACAAAAATGTTCCCAGCGTCACGCTAGGCCGCCTGGCGATAGATAAACGCTTCCAGGGGCAAGGATT
GGGGGAGTTACTGGTCACCCATGCTCTGAAAACGGTCTACCTGGCGTCTTTTGCCGTGGGTATCCACGGGATATTCGTAG
AAGCGCTGAACGACAGCGCCAAAAACTTTTACCTTAAACTGGGGTTTATCGCCCTTCAGGCAGAGAATGAAAATACTCTC
TTCCTGCCGACTAAAACCATTGAAAGACTGTTTGCAGATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10177.54 Da        Isoelectric Point: 5.8652

>AT74030 WP_013095428.1 NZ_CP020089:c693170-692901 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Download         Length: 270 bp

>AT74030 NZ_CP020089:c693170-692901 [Enterobacter cloacae]
ATGTCAGCACTAAAGAAACAGCGCATCGATCTACGCTTAACCGAAGACGACAAAAGCCTGATCGAAGAAGCAGCGGCAAT
GACGAACCAGTCAATTTCACAATTCATGGTAAGTACGGCCTCCGAGCGCGCAGCAGAAGTGATTGAACAGCACCGCCGCC
TGATCCTCAACGAGGCATCCTGGAACCAGGTGATGGATGCGATCAGTAACCCGCCAACGCCAAATGAACGACTGAAGCGA
GCAGCCAGGCGTCTCCAGGATCTGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JFS7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP76

References