Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 580985..581501 Replicon chromosome
Accession NZ_CP020089
Organism Enterobacter cloacae strain PIMB10EC27

Toxin (Protein)


Gene name relE Uniprot ID A0A5E1ALA9
Locus tag B2J95_RS02895 Protein ID WP_038985641.1
Coordinates 581217..581501 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7Z1JC92
Locus tag B2J95_RS02890 Protein ID WP_058651171.1
Coordinates 580985..581227 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2J95_RS02875 576974..577714 + 741 WP_013095353.1 KDGP aldolase family protein -
B2J95_RS02880 577838..578977 + 1140 WP_105826553.1 lactonase family protein -
B2J95_RS02885 578997..580907 + 1911 WP_040020451.1 BglG family transcription antiterminator -
B2J95_RS02890 580985..581227 + 243 WP_058651171.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B2J95_RS02895 581217..581501 + 285 WP_038985641.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B2J95_RS02900 581505..581969 - 465 WP_058651169.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
B2J95_RS02905 582092..584230 - 2139 WP_013095357.1 anaerobic ribonucleoside-triphosphate reductase -
B2J95_RS02910 584604..586247 - 1644 WP_058678894.1 alpha,alpha-phosphotrehalase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10996.90 Da        Isoelectric Point: 10.4610

>T74029 WP_038985641.1 NZ_CP020089:581217-581501 [Enterobacter cloacae]
ITYELEFDPRALKEWSKLGETVKKQFRKKLAGVLVNPRIESARLHNLPDCYKIKLRSQGYRLVYQVQDNVVTVVVIAIGK
REKLTVYHDANKRL

Download         Length: 285 bp

>T74029 NZ_CP020089:581217-581501 [Enterobacter cloacae]
ATAACTTATGAACTGGAATTTGACCCGAGGGCGTTAAAAGAGTGGAGCAAGCTGGGGGAGACTGTAAAAAAGCAGTTCAG
GAAGAAACTTGCCGGGGTGTTGGTAAACCCCCGGATCGAATCGGCCCGTCTGCATAATCTGCCTGATTGCTACAAAATAA
AACTCAGATCCCAGGGTTACCGTTTGGTCTATCAGGTTCAGGACAACGTGGTAACGGTAGTTGTCATCGCTATTGGGAAA
AGAGAGAAATTAACCGTGTATCACGATGCTAACAAACGGCTATAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8971.39 Da        Isoelectric Point: 6.2984

>AT74029 WP_058651171.1 NZ_CP020089:580985-581227 [Enterobacter cloacae]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAETGRMPVKTVTISDSEDALIQTVRERLANPQKGIKVSLDNL

Download         Length: 243 bp

>AT74029 NZ_CP020089:580985-581227 [Enterobacter cloacae]
ATGGCTACGCTTAACGTCCGTCTGGATGACAAACTCAAAAACGAGGCTTATGCCGTGCTGGAAAAACTCAATATCACCCC
AACAGAAGCCGTGCGACTCCTTTTTCAGTACGTTGCGGAGACGGGGCGCATGCCGGTGAAAACGGTCACGATTAGCGACA
GCGAAGATGCGCTGATACAGACAGTCCGGGAGCGACTGGCTAATCCGCAAAAGGGAATTAAGGTTAGTCTGGATAACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1ALA9

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z1JC92

References